奥美沙坦酯对动脉粥样硬化大鼠骨保护素表达的影响

目的探讨奥美沙坦酯对动脉粥样硬化模型大鼠骨保护素、血清炎症因子表达的影响。方法 2015年于江苏省血吸虫病防治研究所收集24只SD大鼠,随机均分为3组。对照组大鼠饲喂普通饲料。模型组大鼠饲喂高脂饲料(普通饲料+0.2%胆酸钠+7×105 IU/(kg·d)维生素D3+2%胆固醇+5%山羊脂肪)。奥美沙坦酯组大鼠饲喂高脂饲料的同时予3mg/(kg·d)奥美沙坦酯灌胃。饲养4周后观察各组大鼠主动脉病理形态学变化,分别检测血清血脂(包括总胆固醇、低密度脂蛋白、高密度脂蛋白、三酰甘油)和血清超敏C反应蛋白水平,免疫组织化学染色和蛋白质免疫印迹(Western blot)法检测骨保护素在主动脉的表达。结果模型组大鼠血清血脂和超敏C反应蛋白水平明显升高,内膜厚度与内膜/中膜厚度比明显升高,主动脉中骨保护素表达较对照组显著升高,差异有统计学意义(P0.05)。奥美沙坦酯组与模型组相比,血清高密度脂蛋白明显升高,其他血清血脂和超敏C反应蛋白水平明显下降,主动脉中骨保护素表达减少,内膜厚度、内膜/中膜厚度比明显降低,差异有统计学意义(P0.05)。结论奥美沙坦酯可能通过改善血脂、降低动脉骨保护素表达和血清炎症因子水平抑制动脉粥样硬化进展。

Effect of olmesartan medoxomil on the expression of osteoprotegerin in rats with atherosclerosis

Objective To investigate the effect of olmesartan medoxomil on osteoprotegerin and serum inflammatory factor expression in atherosclerosis model rat.Methods From 2015 April in Jiangsu Institute of schistosomiasis control,24 Sprague-Dawley rats were randomly divided into the following three groups:the control group was fed with standard food,the model group was fed with high fat diet based on standard food(normal food +2％ cholesterol +5％ goat fat +0.2％ acid +7×105 IU/(kg·d) Vitamin D3),and the olmesartan group was given 3 mg/(kg·d) olmesartan gavage daily on the basis of high fat diet.On the fourth week of experiment the level of serum high sensitivity C reactive protein and serum lipids include total cholesterol,low density lipoprotein,high density lipoprotein and triglyceride were detected.The structure and changes of aortic pathology was observed.The expression of osteoprotegerinin in aortic was detected by immunohistochemistry staining and Western blot.Results Within the model group,the level of serum lipid and high-sensitivity C reactive protein increased significantly,endometrial thickness,intima-to-media thickness ratio and osteoprotegerin expression in aorta was significantly higher than that of normal group,the difference was statistically significant(P<0.05).The serum high density lipoprotein increased significantly,the level of other serum lipids and high sensitive C reaction protein decreased significantly,endometrial thickness,intima-to-media thickness ratio and osteoprotegerin expression in aorta of the olmesartan treated rats were significantly lower than those of model animals,the difference was statistically significant(P<0.05).Conclusion Olmesartan may suppress the development of atherosclerosis in model rats by decreasing the expression of osteoprotegerin and the level of serum inflammatory cytokines.