程序性死亡蛋白配体-1/程序性死亡蛋白-1在老年胃癌患者外周血CD8+T淋巴细胞中的表达及临床意义

目的 通过分析程序性死亡蛋白配体-1/程序性死亡蛋白-1(programmed death ligand-1/programmed death-1,PD-L1/PD-1)在老年胃癌患者外周血CD8+T淋巴细胞中表达情况,探讨其临床意义.方法 选择同一时期90例老年胃癌患者(具有不同临床分期)及90例老年健康体检者,分别采取新鲜外周血后经流式细胞仪检测血中CD8+T淋巴细胞表面PD-L1/PD-1表达情况,结合老年胃癌患者的临床分期,分析PD-L1/PD-1在不同胃癌分期中的表达意义.结果 PD-L1/PD-1在老年胃癌患者外周血CD8+T淋巴细胞呈高表达,相较于老年健康者差异具有统计学意义(t=7.043,P<0.05);外周血CD8+T淋巴细胞的PD-L1/PD-1阳性表达均随着临床分期的增加而增加,呈明显的正相关性(r=0.883,P<0.05);外周血CD8+T淋巴细胞的PD-1阳性表达随着临床分期的增加而增加,两者之间呈正相关性(r=0.811,P<0.05);临床分期较晚的老年胃癌患者外周血CD8+T淋巴细胞PD-L1/PD-1表达比其他相对较早的临床分期老年胃癌患者显著上升(t=4.377,P<0.05).结论 PD-L1/PD-1信号通路异常在老年胃癌患者病变的发生发展过程中发挥了重要作用,外周血CD8+T淋巴细胞PD-L1/PD-1的表达对评判患者的预后具有指导作用.

Expression of programmed death ligand-1/programmed death-1 in peripheral blood CD8+ T lymphocytes in elderly patients with gastric cancer

Objective To analyze the expression of programmed death ligand-1/programmed death-1(PD-L1/PD-1) in peripheral blood CD8+T lymphocytes in elderly patients with gastric cancer and explore its clinical significance.Methods 90 elderly patients with gastric cancer (with different clinical stages) and 90 elderly health examination were selected in the same period.The expression of PD-L1/PD-1 on the surface of peripheral blood CD8+T lymphocytes was detected by flow cytometry.The expression of PD-L1/PD-1 in different stages of gastric cancer was analyzed by the clinical staging of patients with gastric cancer.Results The expression of PD-L1/PD-1 in peripheral blood CD8+ T lymphocytes of patients with gastric cancer was significantly higher than that in elderly healthy person (t=7.043,P<0.05).The positive expression of PD-L1/PD-1 in peripheral blood CD8+ T lymphocytes increased in advanced stage (r=0.883,P<0.05);The positive expression of PD-1 in peripheral blood CD8+ T lymphocytes increased with the clinical stage,there was a positive correlation between the two(r=0.811,P<0.05);The expression of PD-L1/PD-1 in peripheral blood CD8+ T lymphocytes was significantly higher than that of other relatively early clinical stages (t=4.377,P<0.05).Conclusion PD-L1/PD-1 signaling pathway plays an important role in the pathogenesis of gastric cancer in elderly patients.The expression of PD-L1/PD-1 in peripheral blood CD8+ T lymphocytes has a guiding role in judging the prognosis of patients with gastric cancer.