人凋亡相关半胱氨酸肽酶4基因在川崎病中的表达及与核转录因子-κB信号通路的相关性

目的 探讨人凋亡相关半胱氨酸肽酶4基因(Caspase4,Casp4)在川崎病中的表达及与核转录因子-κB(nuclear factor-kappa B,NF-κB)信号通路的相关性.方法 收集上海市第六人民医院东院儿科2012年1月至2015年1月的15例川崎病患者血液样本然后提取单核细胞,采用mRNA定量检测血液中单核细胞Casp4及NF-κB信号通路相关因子表达情况,同时用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)和免疫蛋白印迹方法检测Casp4蛋白及NF-κB信号通路相关因子表达情况,并对结果进行生物统计学的分析.结果 mRNA定量、ELISA和及免疫蛋白印迹方法结果表明川崎病患儿血液CASP4基因表达与健康对照组相比显著升高,NF-κB信号通路相关因子表达也发生变化.数据分析证明川崎病患儿血液中NF-κB p65和Casp4基因mRNA表达情况存在线性关系.结论 婴幼儿在受到环境因素和遗传因素影响后,NF-κB信号通路激活,NF-κB被释放进入细胞核内,引起Casp4基因变化,CASP4基因在川崎病中的高表达及与NF-κB信号通路相关.

The expression of caspase4 gene in Kawasaki disease and its relation with nuclear factor-kappa B signaling pathway

Objective To study the expression of Caspase 4 (Casp4) gene in Kawasaki disease and its relation with nuclear factor-kappa B (NF-κB) signaling pathway.Methods The blood samples of 15 patients with Kawasaki disease from January 2012 to January 2015 in Shanghai No.6 People's hospital East hospital were collected and the peripheral blood mononuclear cells were extracted.The expression of Casp4 gene and NF-κB signaling pathway related factors were detected by mRNA quantitative detection.The expression of Casp4 protein and NF-κB signaling pathway related factors were detected by enzyme-linked immunosorbent assay(ELISA) and Western blotting.Results The results of mRNA quantitative detection,ELISA and Western blotting showed that the expression of Casp4 gene in patient with kawasaki disease was significantly higher than that of healthy controls.NF-κB signaling pathway related factor expression also changed significantly.Data analysis showed that there were a linear relationship between the NF-κB p65 and Casp4 gene mRNA expression in patients with kawasaki disease.Conclusion The NF-κB signaling pathway was activated while infants affected by environmental factors and genetic factors.NF-κB was released into the nucleus,causing the altered expression of Casp4 gene.High expression of Casp4 in patients with kawasaki disease was related to NF-κB signaling pathway.