补肾健脾方治疗环磷酰胺所致小鼠骨髓造血抑制模型的研究

目的通过建立小鼠模型来证实补肾健脾方对环磷酰胺所致骨髓造血抑制的治疗作用。方法将C57BL/6小鼠分为正常对照组、骨髓造血抑制组和补肾健脾方治疗组,骨髓造血抑制组和补肾健脾方治疗组腹膜注射环磷酰胺3d,第4天开始,补肾健脾方治疗组给予中药方剂灌胃治疗,其余两组给予饮水灌胃对照。治疗14d后,利用血细胞分析仪和流式细胞仪分别检测小鼠外周静脉血血细胞计数、骨髓造血干细胞数目和骨髓单个核细胞增殖能力。结果与正常对照组相比,骨髓造血抑制组白细胞计数、骨髓造血干细胞数目、骨髓单个核细胞增殖能力明显下降(P0.01)。经补肾健脾方治疗后,补肾健脾方治疗组血小板计数、骨髓造血干细胞数目、骨髓单个核细胞增殖能力明显高于骨髓造血抑制组(P0.05)。结论补肾健脾方能减轻环磷酰胺化疗的不良反应,提高化疗药物的抗肿瘤效果。

Study on Bushen Jianpi Recipe in treating bone marrow hematopoietic suppression mouse model caused by cyclophosphamide

Objective To verify the effects of Bushen Jianpi Recipe(BSJP) on bone marrow hematopoietic suppression mouse model caused by cyclophosphamide .Methods The experimental C57BL/6 mice were divided into the normal control group ,bone marrow hematopoietic suppression group and BSJP treatment group ,and the bone marrow hematopoietic suppression group and BSJP treatment group were peritoneally injected with cyclophosphamide for 3 d .The BSJP group began to be given with BSJP by gavage from 4 d ,and the other two groups received water as gavage control .After 14 d treatment ,peripheral venous blood cells count ,bone marrow hematopoiesis stem cells (HSCs) count and bone marrow mononuclear cell proliferation ability were detected by using the blood cells analyzer and flow cytometry .Results Compared with the control group ,the white blood cells(WBCs) count , HSCs number and bone marrow mononuclear cell proliferation ability in the hematopoietic suppression group were decreased significantly(P＜0 .01);the platelet count ,HSCs count and bone marrow mononuclear cells proliferation ability after BSJP treatment in the BSJP treatment group were significantly higher than those in the hematopoietic suppression group (P＜0 .01) .Conclusion BSJP can alleviate the side effect of cyclophosphamide chemotherapy and increase the anti-tumor effect of chemotherapeutic drugs .