血管内皮生长因子-D、 微血管密度及微淋巴管密度的表达与乳腺癌临床病理特征的相关性

目的 探讨乳腺癌中血管内皮生长因子D(vascular endothelial growth factor-D,VEGF-D)及微血管密度(microvessel density,MVD)、微淋巴管密度(Lymphatic vessel density,MLD)表达情况及意义.方法 收集2009年1月至2016年5月在临沂市肿瘤医院病理科存档病例,其中乳腺癌61例,乳腺增生症23例.采用免疫组化法检测各组织VEGF-D、MVD和MLD表达.结果 乳腺癌组织中VEGF-D蛋白阳性率为62.30%,明显高于乳腺增生组织,差异有统计学意义(χ2=16.215,P<0.05);乳腺癌组织中MVD和MLD分别为(17.70±7.10)和(2.41±0.85),明显高于乳腺增生组织,差异有统计学意义(t=10.900、t=8.795,P<0.05);VEGF-D表达与乳腺癌患者年龄、肿瘤大小以及淋巴结转移无明显关系(χ2=0.394、0.032、0.244,P>0.05);MVD和MLD密度与乳腺癌患者年龄和肿瘤大小无明显差异(χ2=0.081,0.126,0.219,0.196,P>0.05);VEGF-D、MVD和MLD密度与乳腺癌组织学分级相关(χ2=13.076、23.892、10.082,P<0.05),随着组织学分级递增,VEGF-D、MVD和MLD密度明显增高;MVD和MLD在乳腺癌伴淋巴结转移中密度明显高于无淋巴结转移(t=2.481、5.791,P<0.05).结论 VEGF-D在乳腺癌组织中呈高表达,可能在乳腺癌的发生发展中起重要作用,而MVD和MLD与乳腺癌肿瘤分化、转移有一定的关系.

The expression of vascular endothelial growth factor-D,microvessel density,and micro lymphatic vessel density and its correlation with breast cancer

Objective To investigate the expression of vascular endothelial growth factor D (VEGF-D),microvessel density(MVD),and micro lymphatic vessel density (MLD) in breast cancer and its significance.Methods From January 2009 to May 2016 breast tissues enbedded in paraffin were collected.These tissues include 61 cases of breast cancer and 23 cases of cyclomastopathy.The expression of VEGF-D,MVD,and MLD were detected by immunohistochemistry.Results The positive rate of VEGF-D in breast cancer tissues was 62.30%,which was significantly higher than that of the hyperplasia tissue of mammary gland (χ2=16.215,P< 0.05).MVD and MLD in breast cancer tissues were 17.70±7.10 and 2.41±0.85 respectively,There are also significantly higher than that in the hyperplasia tissue of mammary gland(t=10.900,8.795,χ2=16.125,P<0.05).VEGF-D expression was not significantly associated with age,tumor size,and lymph node metastasis in breast cancer patients (χ2=0.394,0.032,0.244,P> 0.05).The expression of MVD and MLD had no significant relationship with age and tumor size in patients with breast cancer (χ2=0.081,0.126,0.219,0.196,P> 0.05).The expression of VEGF-D,MVD and MLD was related to the histological grade of breast cancer (χ2=13.076,23.892,10.082,P<0.05).The expression of VEGF-D,MVD,and MLD was significantly increased in cancer tissues with high histological grade.The expression of MVD and MLD in breast cancer with lymph node metastasis was significantly higher than that without lymph node metastasis (t=2.481,5.791,P<0.05).Conclusion VEGF-D is highly expressed in breast cancer,which may play an important role in the development of breast cancer.MLD and MVD also contribute to the differentiation and metastasis of breast cancer.