肺结核患者外周血不同T淋巴细胞亚群中白细胞介素-9产生细胞比例的研究

目的 探讨肺结核病(tuberculosis,TB)患者外周血不同T淋巴细胞亚群中白细胞介素(interluekin,IL)-9产生细胞比例的变化.方法 采集26例活动期肺TB患者(TB组)和23例健康志愿者(HD组)外周血样本,加刺激剂佛波醇酯(PMA)和钙离子霉素(IM),以及阻断剂莫能霉素作用6h后,用荧光标记单克隆抗体作T细胞亚型表面分子和胞内细胞因子染色,流式细胞仪检测不同T淋巴细胞亚群中分泌IL-9细胞的比例.结果 CD3+总T细胞中产生IL-9的细胞比例在TB组和HD组分别为0.32％和0.35％,在CD4+ αβ T细胞中分别为0.21％和0.22％,在CD8+ αβ T细胞中分别为0.35％和0.32％,差异均无统计学意义(P＞0.05).而在γδ T细胞中产生IL-9细胞的比例,TB组(2.93％)高于HD组(1.07％),差异有统计学意义(P＜0.05).结论 与HD组比较,TB患者CD4+T细胞和CD8+T细胞中IL-9产生细胞的比例无明显改变,而γδ T细胞中分泌IL-9细胞比例显著增加.

Study of interleukin-9 producing cell proportion in different T lymphocyte subpopulations in peripheral blood in patients with pulmonary tuberculosis

Objective To explore the changes of the proportion of (interleukin,IL)-9 producing T cells among different T cell subpopulations of peripheral blood from patients with active pulmonary tuberculosis (TB).Methods Peripheral blood samples were obtained from 26 pulmonary TB patients and 23 health donors,and stimulated with Phorbo 12-myristate 13-acetate (PMA) and ionomycin (IM) for 6 h in the presence of monensin (Mo) at last 4h.Cells were collected and stained with fluorochrome conjugated mAbs,to cell surface makers for phenotypes and to intracellular cytokine.The proportions of IL-9 producing cells in different T cell subpopulations were detected by flow cytometry.Results The proportions of IL-9 producing cells among total CD3 + T cells in TB patients and health donors were 0.32％,and 0.35％,respectively,and that among CD4 + αβ T cells were 0.21％ and 0.22％,respectively;and that among CD8 + αβ T cells were 0.35％ and 0.32％ respectively;there were no significant differences in two groups.Whereas,the percentage of IL-9 producing cells in γδ T cells in TB patients (2.93％) was significantly higher than that in healthy donors group(1.07％) (P ＜ 0.01).Conclusion Compared to healthy donors group,there is no significant change of IL-9 producing cell proportions in CD4 and CD8αβ T cells in TB patients.However,there is increase of IL-9 producing γδ T cells in peripheral blood of TB patients.