特发性血小板减少性紫癜患者T细胞及B细胞亚群变化的研究

目的 探讨特发性血小板减少性紫癜(idiopathic thrombocytopenic purpura,ITP)患者T、B淋巴细胞亚群的变化.方法 从大理州人民医院收集ITP患者和健康者的外周血,用Sysmex血液分析仪及流式细胞术分析T细胞及B细胞亚群.结果 与健康人群比较,ITP患者CD3+T细胞百分率无明显变化,CD4+T细胞百分率降低(42.39％±12.12％,P＜0.05),CD8+T细胞百分率升高(46.93％±11.99％,P＜0.05);CD19+B细胞百分率升高(14.11％±10.28％,P＜0.05),T2B细胞百分率(34.51％±9,70％,P＜0.05)、成熟B细胞百分率(30.91％±7.12％,P＜0.05)及记忆性B细胞百分率(23.31％±8.23％,P＜0.05)增多,差异均有统计学意义.ITP患者T1 B细胞、Kappa+B细胞、Lambda+B细胞百分率与健康人差异无统计学意义.结论 ITP患者外周血T细胞亚群和B细胞亚群分布发生了变化,这种变化在ITP发病中的作用尚需进一步研究.

T and B lymphocyte subsets in idiopathic thrombocytopenic purpura patients

Objective To investigate the changes of T cell and B cell subsets in idiopathic thrombocytopenic purpura(ITP) patients.Methods T cells and B cells and their subsets in the peripheral blood from ITP patients and healthy volunteers in a hospital of Dali were analyzed with Sysmex hematology analyzers and flow cytometry.Results Compared with the healthy population,the results show that the percentage of CD3 + T cells had no significant change and CD4 + T cells reduced (42.39％ ± 12.12％,P ＜ 0.05),but CD8 + T ceils increased(46.93％ ± 11.99％,P＜0.05).CD19 +B cells (14.11％ ± 10.28％,P＜0.05),T2 B cells (34.51％ ±9.70％,P ＜0.05),mature B cells (30.91％ ±7.12％,P ＜0.05) and memory B cells increased (23.31％ ± 8.23 ％,P ＜ 0.05) in ITP patients.The differences were statistically significant.T1 B cells,Kappa + B cells,Lambda + B cells show no significant change in ITP patients.Conclusion Preliminary studies have shown that some T cell subsets and B cell subsets of ITP patients were changed,and the role of these changes in ITP requires further study.