Bilateral electroretinographic changes induced by unilateral intra—visual cortex inoculation of herpes simplex virus type 1 in BALB/c mice

Intra—visual cortex inoculation of 10² plaque—forming units of herpes simplex virus type 1 (KOS—63) induced physiologic and morphologic retinal changes in 62.3% (33/53) of infected animals; of these, 91% were bilateral. In contrast, inoculation of the same viral titers into the frontal lobe induced retinal alterations in only 13.3% (2/15). Initially, there was a decrease of the b—wave amplitude and retinal sensitivity and necrotic changes of the ganglion cells and nuclei in the inner nuclear layer. Immunoperoxidase staining for virus—specific antigens showed positive staining of the same cell type. Over time, there was a progressive decrease in the electroretinogram until it was extinguished and the retina was replaced by gliotic tissue. Parallel viral recovery studies demonstrated detectable infectious virus in one of eight eyes on day 2 after inoculation and in three of eight eyes on day 4. Thereafter, there was an increase in the percentage of eyes with infectious virus and a concomitant increase in viral titers. Immunoperoxidase staining of brain sections obtained on days 6 through 8 demonstrated virus—specific antigens on cells in the lateral geniculate nuclei and the suprachiasmatic nuclei bilaterally.Abbreviations HSV herpes simplex virus - IVC intra—visual cortex