人血管内皮细胞氧化低密度脂蛋白基因表达谱分析

目的 检测内皮细胞中与低密度脂蛋白氧化可能相关的信号传导相关蛋白基因,期望获得阐明氧化机制的有价值的线索.方法 利用低密度脂蛋白作用人血管内皮细胞系ECV304,通过基因芯片分析人信号传导相关蛋白的表达谱.结果 在被检测的1 990个基因中,52个基因的表达变化超过了2倍以上,其中23个基因表达增加,29个基因表达减少.在表达发生改变的基因中,有7个已在最新的报道中认为可能和动脉粥样硬化相关.结论 我们的发现可能给内皮细胞氧化低密度脂蛋白的机制的揭示提供全新的思路.

Expression Profiling Analysis of ECV304 Endothelial Cells Induced by Low Density Lipoprotein

Aim To determine the genes involved in the mechanisms of LDL oxidation mediated by endothelial cells. Methods Microarray analysis on human signal transduction associated proteins was applied to profile changes in gene expression of human ECV304 endothelial cells induced by low density lipoprotein. Results In 1 990 genes detected,52 genes were identified to be >2-fold difference in expression, among which 23 genes with elevated expression and 29 genes with reduced expression. Some of these 52 genes encoding proteins have been determined to be correlated with atherosclerosis. Conclusion Those genes expressed differently may provide new insights into the possible mechanisms of low density lipoprotein oxidation mediated by endothelial cells.