甘肃省长江流域地区蚊虫及其携带病毒调查

目的了解甘肃省长江流域地区蚊虫分布特点及所携带病毒情况,为当地防治虫媒病毒相关疾病提供科学依据。方法使用电动吸蚊器、捕虫网捕捉成蚊,并进行分类。用细胞培养法分离病毒,用血清学及分子生物学方法进行病毒鉴定。结果 2006-2009年共捕获蚊虫10528只,包括5属30种,其中三带喙库蚊5981只,占捕蚊总数的56.81%;骚扰阿蚊、中华按蚊、淡色库蚊、白纹伊蚊分别占33.36%、4.95%、3.29%和0.95%,待定种占0.64%。从蚊虫中分离到1株流行性乙型脑炎病毒（JEV）,1株版纳病毒（BAV）和15株未知RNA病毒。结论三带喙库蚊是甘肃省长江流域地区的优势蚊种,蚊虫携带JEV和BAV等虫媒病毒,应在该地区加强相关蚊虫及虫媒病毒的调查及防治工作。

Investigation of mosquitoes and mosquito-borne viruses in the Yangtze River Basin within Gansu province

Objective To determine the distribution of mosquitoes and mosquito-borne viruses in the Yangtze River Basin within Gansu province,providing the basis for prevention and control of arbovirus-related diseases.Methods Electric mosquito-suction devices and insect-catching nets were employed to capture adult mosquitoes.The captives were classified and subject to virus isolation using cell culture.Serologic and molecular methods were used for virus identification.Results From 2006 to 2009,10 528 mosquitoes were captured in the Yangtze River Basin,including 30 species of 5 genera.Among them were 5981 Culex tritaeniorhynchus,accounting for 56.81% of the total captives;Armigeres subalbatus,Anopheles sinensis,Cx.pipiens pallens and Aedes albopictus accounted for 33.36%,4.95%,3.29% and 0.95%,respectively;and unidentified species accounted for 0.64%.A Japanese encephalitis virus（JEV）,a Banna virus（BAV） and 15 unidentified RNA viruses were isolated from the mosquitoes.Conclusion Cx.tritaeniorhynchus was the predominant mosquito species in the Yangtze River region within Gansu province.Since the mosquitoes were carrying such arbovirus as JEV and BAV,it should be strengthen the investigation,prevention and control of the related mosquitoes and arboviruses in the region.