胎盘免疫调节因子对大鼠子宫内膜异位症的疗效观察

目的 研究胎盘免疫调节因子(placental immunoregulatory factor,PF)对大鼠子宫内膜异位症(endometriosis,EMS)的治疗效果.方法 建立雌性大鼠EMS模型,随机分为对照组、模型组、PF低、中、高治疗组.PF治疗8周后开腹测量异位内膜生长体积;用淋巴细胞非特异性酯酶(achroacyte acor nonspecific esterase,ANAE)组织化学染色方法检测用药后外周血ANAE阳性细胞;用ELLSA法测量血清中细胞因子白细胞介素-2(interleukin-2,IL-2)含量.结果 各治疗组治疗后异位内膜体积较治疗前小(P＜0.05);PF能明显提高各治疗组ANAE活性(P＜0.05)和血清IL-2含量(P＜0.05).结论 PF可以提高机体的细胞免疫功能,对大鼠EMS模型的生长及粘连起到抑制或治疗作用.

THERAPEUTIC EFFECT OF PLACENTAL IMMUNOREGULATORY FACTOR ON EXPERIMENTAL ENDOMETRIOSIS IN RATS

Objective To investigate the treatment effects of placental immunoregulatory factor on the experimental endometriosis in rats.Methods The rats with experimental endometriosis were randomly divided into 5 groups: control,model,and three treatment groups with placental immunoregulatory factor.After eight weeks of treatment with placental immunoregulatory factor,diameters of implants were measured and the morphological changes observed,achroacyte acor nonspecific esterase(ANAE) activity in peripheral blood were detected with histochemical method endometriosis.And serum interleukin-2(IL-2) was determined by ELLSA.Results After treatment,the volume of the ectopic implants in each treatment group was significantly deoreased.Placental immunoregulatory factor could obviously increase ANAE activity and level of IL-2.Conclusion Placental immunoregulatory factor can increase cellular immune function of the rat with endometriosis.The right dosage of placental immunoregulatory factor could play inhibition or treatment role on experimental endometriosis in rats.