| 一起麻疹爆发的病毒分离及基因型鉴定 |
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| 引用本文： | 詹军,陈慧,孙晓强,韩玉霞,姬奕昕.一起麻疹爆发的病毒分离及基因型鉴定[J].中国计划免疫,2006,12(5):364-365. |
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| 作者姓名： | 詹军 陈慧 孙晓强 韩玉霞 姬奕昕 |
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| 作者单位： | 宁夏回族自治区疾病预防控制中心 银川750004(詹军，陈慧，孙晓强，韩玉霞)，中国疾病预防控制中心病毒病预防控制所 北京100050(姬奕昕) |
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| 摘 要： | 目的了解宁夏回族自治区流行麻疹野病毒的基因型。方法2004年5月,青铜峡市某小学发生麻疹爆发,收集了3份病人急性期咽拭子标本和11份血标本,分离咽拭子中的麻疹病毒,使用逆转录-聚合酶链反应检测分离到的麻疹病毒核蛋白(N)基因,对扩增后的N基因片段进行核苷酸序列测定,并分析鉴定基因型。结果宁夏回族自治区首次分离到2株麻疹病毒;酶联免疫吸附试验捕捉法检测病人血清的IgM抗体,阳性率为100%;序列分析结果表明,这2株麻疹病毒均属于H1基因型。结论2004年引起青铜峡市麻疹流行的病毒为H1基因型。
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| 关 键 词： | 麻疹病毒 病毒分离 逆转录-聚合酶链反应 基因型 |
| 文章编号： | 1006-916X(2006)05-0364-02 |
| 修稿时间： | 2005年6月17日 |
Virus Isolation and Genotyping From a Measles Outbreak |
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| ZHAN Jun,CHEN Hui,and SUN Xiao-qiang,et al..Virus Isolation and Genotyping From a Measles Outbreak[J].Chinese Journal of Vaccines and Immunization,2006,12(5):364-365. |
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| Authors: | ZHAN Jun CHEN Hui and SUN Xiao-qiang |
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| Abstract: | Objective In order to know the genotype of measles wild virus epidemic in Ningxia.Methods Three throat swab specimens and 11 blood specimens were collected from suspected measles cases of a school of Qingtongxia City,Ningxia in May,2004.Using virus isolation method isolated MV from throat swab specimens.N gene of isolated MV strains was amplified by RT-PCR.The amplified N gene segment was sequenced and analyzed genotype.Results We isolated 2 MV epidemic strains in Ningxia at first time.Positive rate of MV-IgM antibody of patients detected by ELISA capture method was 100%.Sequence analysis indicated that all the 2 epidemic strains belong to H_1 genotype. Conclusion It was H_1 genotype MV that caused measles outbreak in a school of Qingtongxia City,Ningxia in 2004. |
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| Keywords: | Measles virus Virus isolation RT-PCR Genotype |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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