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| The Immunity Induced by Recombinant Spike Proteins of SARS Coronavirus in Balb/c Mice | |
| 作者姓名： | 秦莉 王西明 吴少庭 袁仕善 黄达娜 雷明军 潘辉榕 林绮萍 |
| 作者单位： | Department of Biochemistry and Molecular Biology Tongji Medical College Huazhong University of Science and Technology，Department of Biochemistry and Molecular Biology Tongji Medical College Huazhong University of Science and Technology，Shenzhen Center for Disease Control and Prevention，Shenzhen Center for Disease Control and Prevention，Shenzhen Center for Disease Control and Prevention，Department of Biochemistry and Molecular Biology Tongji Medical College Huazhong University of Science and Technology，Department of Biochemistry and Molecular Biology Tongji Medical College Huazhong University of Science and Technology，Veterinarian College South China University of Agriculture，Wuhan 430030 China，Wuhan 430030 China，Shenzhen 518020 China，Shenzhen 518020 China，Shenzhen 518020 China，Wuhan 430030 China，Wuhan 430030 China，Guangzhou 510000 China |
| 摘 要： | The immune effect of two recombinant protein fragments of spike protein in severe acute respiratory syndrome coronavirus (SARS CoV) was investigated in Balb/c mice. Two partial spike gene fragments S1 (322-1464 bp) and S2 (2170-2814 bp) of SARS coronavirus were amplified by RT-PCR, and cloned into pET-23a prokaryotic expression vector, then transformed into competent Escherichia E.coli BL21 (DE3)(pLysS) respectively. Recombinant proteins were expressed and puri- fied by Ni2 immobilized metal ion affinity chromatography. The purified proteins mixed with com- plete Freund adjuvant were injected into Balb/c mice three times at a two-week interval. High titer antibody was detected in the serum of immunized Balb/c mice, and mice immunized with S1 protein produced high titer IgG1, IgG2a, IgG2b and IgG3, while those immunized with S2 protein produced high titer IgG1, IgG2a, but lower titer IgG2b and IgG3. Serum IFN-γ concentration was increased significantly but the concentrations of IL-2, IL-4 and IL-10 had no significant change. And a marked increase was observed in the number of spleen CD8 T cells. The results showed that recombinant proteins of SARS coronavirus spike protein induced hormonal and cellular immune response in Balb/c mice. |
| 关 键 词： | IFN-γ |
| 收稿时间： | 24 May 2006 |
The immunity induced by recombinant spike proteins of SARS coronavirus in Balb/c mice |
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| Qin?Li,Wang?Ximing,Wu?Shaoting,Yuan?Shishan,Huang?Dana,Lei?Mingjun,Pan?Huirong,Lin?Qiping.The Immunity Induced by Recombinant Spike Proteins of SARS Coronavirus in Balb/c Mice[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(3):221-224. | |
| Authors: | Qin Li Wang Ximing Wu Shaoting Yuan Shishan Huang Dana Lei Mingjun Pan Huirong Lin Qiping |
| Institution: | 1. Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China 2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China 3. Veterinarian College, South China University of Agriculture, Guangzhou 510000, China |
| Abstract: | Summary The immune effect of two recombinant protein fragments of spike protein in severe acute respiratory syndrome coronavirus (SARS CoV) was investigated in Balb/c mice. Two partial spike gene fragments S1 (322 1464 bp) and S2 (2170 2814 bp) of SARS coronavirus were amplified by RT-PCR, and cloned into pET-23a prokaryotic expression vector, then transformed into competent Escherichia E. coli BL21 (DE3)(pLysS) respectively. Recombinant proteins were expressed and purified by Ni2+ immobilized metal ion affinity chromatography. The purified proteins mixed with complete Freund adjuvant were injected into Balb/c mice three times at a two-week interval. High titer antibody was detected in the serum of immunized Balb/c mice, and mice immunized with S1 protein produced high titer IgG1, IgG2a, IgG2b and IgG3, while those immunized with S2 protein produced high titer IgG1, IgG2a, but lower titer IgG2b and IgG3. Serum IFN-concentration was increased significantly but the concentrations of Il-2, IL-4 and IL-10 had no significant change. And a marked increase was observed in the number of spleen CD8+ T cells. The results showed that recombinant proteins of SARS coronavirus spike protein induced hormonal and cellular immune response in Balb/c mice. QIN Li, female, born in 1976, Doctor in Charge |
| Keywords: | severe acute respiratory syndrome coronavirus spike protein IgG IgG1 IgG2a IgG2b IgG3 IL-2 IL-4 IL-10 T lymphocyte subset |
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