氟化钠及其拮抗剂硫酸锌对成骨细胞的影响

目的观察不同剂量氟化钠（ＮａＦ）对成骨细胞的影响及硫酸锌（ＺｎＳＯ４）的拮抗作用，为临床治疗提供实验依据。方法取新生２４ｈＳＤ鼠头盖骨分离成骨细胞，在不同浓度的ＮａＦ和／或ＺｎＳＯ４（１０＾－５ｍｏｌ／Ｌ）中培养。经ＭＴＴ法测定细胞增殖，细胞分化由碱性磷酸酶（ＡＬＰ）和骨钙素水平测定得到。结果ＮａＦ对成骨细胞增殖及分化呈双向调节，主要表现为大剂量抑制，小剂量促进。当ＮａＦ浓度为１０＾－６ｍｏｌ／Ｌ

Effect of sodium fluoride and its antagonist zine sulfate on rat osteoblasts

Objective\ To assess the effect of sodium fluoride(NaF)on rat osteoblasts and the antagonism of zinc sulfate(ZnSO 4)on NaF.Methods\ Osteoblasts obtained from the calvariae of newborn rats were cultured in medium with different concentrations of NaF and /or ZnSO 4.Cell proliferation was detected by MTT method and differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion.Results NaF increased cell proliferation and differentiation in a double regulation manner,i.e.low doses of NaF stimulated both proliferation and ALP activity of osteoblasts,while high doses suppressed them. However,all doses of NaF we used increased osteocalcin production.ZnSO 4 was synergistic with low doses of NaF and antagonistic to high doses of NaF by restoring partly the suppressive effect of NaF on cell proliferation and ALP activity,as well decreasing the enhancement of osteocalcin secretion by NaF. Conclusion NaF acts on osteoblast proliferation in a double regulated manner and possesses multiple effects on cell differentiation.10 -5 M ZnSO 4 antagonizes high doses of NaF,but does not antagonizes low doses of NaF.