高效毛细管电泳法测定肤炎康霜中地塞米松磷酸钠的含量

目的 测定肤炎康霜中地塞米松磷酸钠的含量。方法 采用高效毛细管电泳法。毛细管60 cm×75μm、运行缓冲液100 mmol/L四硼酸钠(pH 9.2)、高压进样10s、分离电压25kv、温度为25℃、检测波长为240 nm。结果 地塞米松磷酸钠在20～100 μg/ml浓度范围内线性关系良好(r=0.9998),平均回收率为99.75%(n=5,RSD=1.03%)。结论 本法简单、快捷、灵敏,可用于肤炎康霜的质量控制。

Determination of dexamethasone sodium phosphate content in Fuyankang cream by high-performance capillary electrophoresis

Objective To determine the content of dexamethasone sodium phosphate in Fuyankang cream, a preparation for treatment of various skin conditions. Methods Dexamethasone sodium phosphate content in Fuyankang cream was determined using high-performance capillary electrophoresis (HPCE) at the detection wavelength of 254 nm, under the optimal condition achieved with a fused-silica capillary tube (60 cm×75 μm) and 100 mmol/L sodium tetraborate buffer (pH 9.2) at a constant voltage of 25 kV with a sampling time of 10 s at 25℃. Results The calibration curve displayed good linear relationship within dexamethasone sodium phosphate concentration range of 20 to 100μg/ml (r=0.999 8). The average recovery of dexamethasone sodium phosphate was 99.75% (n=5, RSD=1.03%). Conclusion HPCE is simple, quick and sensitive in determination of dexamethasone sodium phosphate content in Fuyankang cream.