豨莶液的定性定量测定方法

目的：建立控制豨莶液质量的方法。方法：采用HPLC法定量测定豨莶液中的奇任醇含量。色谱条件：色谱柱为Allsphere ODS（4.6mm×250mm,5μm）,流动相为乙腈-水-醋酸-三乙胺（25∶75∶0.1∶0.2）,流速为1.0mL/min,检测波长为215nm,进样量为10μL,柱温为20℃。定性鉴别采用薄层色谱法。结果：在本色谱条件下,奇任醇在0.4168-1.6672μg范围内呈良好的线性关系。平均回收率为99.78%（RSD=1.51%,n=6）。薄层色谱中豨莶草的色谱斑点清晰、无干扰。结论：本法定性定量结合,准确可靠,重现性好,快速简便,可用于豨莶液的质量控制。

The Method of Quality Control for A Formulation of Traditional Chinese Medicine "Xixian Ye"

Objective： To establish a method lation of Traditional Chinese Medicine made of of controlling the quality for ＂Xixian Ye＂ that is a formuHerba Siegesbeckiae. Methods： HPLC was adopted for determining the Kirenol content of this formulation, in which Allsphere ODS C18 column （4.6 mm × 250 mm, 5 μm） and acetonitrile-water-acetic acid-triethylamine（25： 75： 0.1：0.2） were used as the mobile phase at a flow rate of 1.0 mL/min, with a detection wavelength of 215 nm and a injection volume of 10μL and column temperature of 20 ℃. Moreover, TLC was adopted for qualitative identification of ＂Xixian Ye＂. Results： Under these chromatographic conditions, a good linearity relationship with the peak areas was exhibited （r = 0. 999 5） while the sample size of Kirenol was in the range of 0.416 8 - 1. 667 2 μg. The average recovery of Kirenol was 99.78 % （ RSD = 1.51%, n = 6）. And a legible spot of Herba Siegesbeckiae was got by TLC. Conclusion： This method is accurate and reliable. It can effectively control the quality of the Chinese Traditional Medicine Formulation ＂Xixian Ye＂.