| 调节性T细胞及Foxp3基因在再生障碍性贫血患儿外周血中的变化及意义 |
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| 引用本文： | 王西阁,王晓格,栾斌,胡姬婷.调节性T细胞及Foxp3基因在再生障碍性贫血患儿外周血中的变化及意义[J].中国当代儿科杂志,2010,12(4):241-243. |
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| 作者姓名： | 王西阁 王晓格 栾斌 胡姬婷 |
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| 作者单位： | 王西阁，王晓格，栾斌，胡姬婷 |
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| 基金项目： | 河南省科技厅基金资助项目(0624410046) |
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| 摘 要： | 目的：研究CD4+CD25+CD127low调节性T细胞(Treg)及Foxp3基因在再生障碍性贫血（简称再障）患儿外周血中的表达水平，探讨其在再障发病机制中的作用。方法：采用流式细胞术分别检测21例慢性再障(CAA)、9例急性再障(SAA)和15例健康儿童（对照组）外周血Treg细胞的比例变化，并用RT-PCR方法检测其外周血中Foxp3 mRNA的表达水平。结果：SAA和CAA组患儿外周血中CD4+T细胞百分比、CD4+CD25+、CD4+CD25+CD127lowTreg细胞占CD4+T细胞百分比均低于对照组（P＜0.05），且SAA组低于CAA组（P＜0.05）；相关基因FoxP3 mRNA在SAA患儿外周血中呈低表达（0.47±0.08）%，明显低于对照组(0.71±0.12)%和CAA组(0.68±0.14)%,P＜0.05。结论：再障患儿外周血Treg细胞和Foxp3 mRNA表达减低，可能在再障的发病过程中有重要作用，且SAA表达低于CAA，有望用于再障病情严重程度的评估。［中国当代儿科杂志，2010，12（4）：241-243］
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| 关 键 词： | 再生障碍性贫血 调节性T细胞 流式细胞术 Foxp3 儿童 |
Expression of regulatory T cells and Foxp3 gene in peripheral blood of children with aplastic anemia |
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| WANG Xi-Ge,WANG Xiao-Ge,LUAN Bin,HU Ji-Ting.Expression of regulatory T cells and Foxp3 gene in peripheral blood of children with aplastic anemia[J].Chinese Journal of Contemporary Pediatrics,2010,12(4):241-243. |
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| Authors: | WANG Xi-Ge WANG Xiao-Ge LUAN Bin HU Ji-Ting |
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| Institution: | WANG Xi-Ge, WANG Xiao-Ge, LUAN Bin, HU Ji-Ting |
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| Abstract: | Objective To investigate the levels of CD4+CD25+CD127low regulatory T cells (Tregs) and the expression of Foxp3 gene in peripheral blood of children with aplastic anemia (AA) and to study their roles in the pathogenesis of AA.Methods Twenty-one children with chronic AA,9 with acute AA and 15 healthy children were enrolled.The proportion of CD4+CD25+ CD127low Tregs in CD4+ T cells was evaluated by flow cytometric analysis.The level of Foxp3 mRNA was ascertained by RT-PCR.Results The percentage of peripheral ... |
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| Keywords: | Aplastic anemia Regulatory T cell Flow cytometry Foxp3 Child |
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