SV40T介导的人胎儿永生化气管成纤维细胞系的建立 |
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引用本文: | 杨梅英,叶常青.SV40T介导的人胎儿永生化气管成纤维细胞系的建立[J].癌变.畸变.突变,1999,11(1):22-24. |
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作者姓名: | 杨梅英 叶常青 |
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作者单位: | 北京放射医学研究所 |
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摘 要: | 建立永生化气管成纤维细胞系为研究细胞恶性转化提供实验模型。用pZIPSV40T转染原代人胎气管成纤维细胞,G418筛选,挑取阳性克隆传代培养,并进行细胞生物学特性鉴定。结果表明,细胞经pZIPSV40T转染后,形态较正常细胞略短,排列不规则,当传到50代时仍生长良好。Southern blot 证实此转化细胞整合了SV40T基因。永生化细胞接种的第4天细胞为指数生长期,染色体核型以非整倍体为主,不
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关 键 词: | 永生化 气管 成纤维细胞 转化 细胞系 |
ESTABLISHMENT OF HUMAN FETAL TRACHEAL FIBROBLAST CELL LINE IMMORTALIZED BY SV40T |
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Abstract: | The aim is immortalizing the human fetal tracheal fibroblast cells to study the cell neoplastic transformation. The primary human fetal tracheal fibroblast cell was transfected with pZIPSV40T vector. The colonies that resistant to G418 were collected, and passaged to immortalize. The biological characters immortalized cell had been determined. Result: the immortalized cell morphology is shorter than normal, arrange un regular. The cells grow well at p50. Southern blot confirmed the presence of T antigen as integrated form in immortalized cell DNA. The index of growth period was fourth day after plated. All the cells could not form colony in soft agar. No tumors formed after SC injection of the cells in nude mice. The result confirmed that human fetal tracheal fibroblast cell can be immortalized by SV40T gene. |
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Keywords: | Immortalization Human fetal fibroblast cell Transformation |
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