反复呼吸道感染患儿T细胞功能性极化状态

目的观察反复呼吸道感染患儿间歇期外周血T细胞功能性极化状态。方法以反复呼吸道感染患儿外周血及健康儿童外周血为研究对象,采用全血PHA刺激培养,四色荧光标记流式细胞仪检测Th1(Tc1)及Th2(Tc2)百分比。结果反复呼吸道感染患儿Th1较正常降低(P<0.05),Th2升高、Th1/Th2比值下降(P<0.01);Tc1降低、Tc2升高、Tc1/Tc2比值下降(P>0.05)。结论反复呼吸道感染患儿间歇期存在Th功能性极化异常,Th2占优势;Tc功能性极化无明显异常。

Functional polarization of T cells in patients with recurrent respiratory tract infections

Objectives To observe the functional polarization of T cells in children with recurrent respiratory tract infections(RRTI)during remission intervals.Methods The serum levels of Th1 and Th2 cytokines in children with RRTI and controls were determined by PHA-stimulated blood culture and 4-color flow cytometry.Results The serum level of Th1 cytokines was lower in children with RRTI than that of the controls(P < 0.05=while the serum level of Th2 cytokines was higher in children with RRTI than that of the controls(P < 0.01=,which made the Th1/Th2 ratio lower in RRTI children than the controls(P < 0.01=.There was no significant difference between the RRTI children and the controls in the decrease of Tc1 cell number,the increase of Tc2 cell number,and the decrease of Tc1/Tc2 ratio.Conclusions Functional polarization of T help cells was abnormal in RRTI children during remission intervals,accompanied by the predomination of Th2 cells and the normal Tc cell polarization.