| 扶正化瘀方对大鼠肾间质纤维化肾组织基质金属蛋白酶活性调节的作用 |
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| 引用本文： | 谢红东,杨珂,王清兰,陶艳艳,刘成海. 扶正化瘀方对大鼠肾间质纤维化肾组织基质金属蛋白酶活性调节的作用[J]. 中华中医药杂志, 2010, 0(10) |
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| 作者姓名： | 谢红东 杨珂 王清兰 陶艳艳 刘成海 |
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| 作者单位： | 上海中医药大学附属曙光医院肝病研究所;上海高校中医内科学E-研究院;上海中医药大学肝肾疾病病证教育部重点实验室; |
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| 基金项目： | 教育部博士点基金资助项目(No.20060268005); 上海市优秀学科带头人计划A类(No.08XD14041); 上海市教育委员会E-研究院建设计划项目(No.E0308)~~ |
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| 摘 要： | 目的:探讨扶正化瘀方影响肾间质纤维化大鼠肾组织基质金属蛋白酶(MMPs)活性调节的抗肾纤维化作用机制。方法:大鼠分为正常组、模型组与扶正化瘀方组,以氯化汞灌胃诱导大鼠肾间质纤维化模型,扶正化瘀方组以4g/kg扶正化瘀方灌胃。生化检测血清肾功能、肾组织羟脯氨酸(Hyp)含量;病理染色观察肾组织病理形态改变;蛋白印迹法分析肾组织MMP-13、MMP-2、膜型基质金属蛋白酶-1(MT1-MMP)及金属蛋白酶组织抑制剂(TIMP-1/2)蛋白表达;明胶酶谱法测定MMP-2活性;荧光底物反应法检测MMP-13活性。结果:与模型组比较,扶正化瘀方组明显改善大鼠血清肾功能指标,降低肾组织Hyp含量,减轻肾组织胶原沉积,降低大鼠肾组织的MMP-2、MT1-MMP、TIMP-1/2蛋白表达,降低MMP-2活性,但增加MMP-13活性。结论:扶正化瘀方抗肾间质纤维化的作用机制在于:抑制MMP-2活性,保护肾组织基底膜;抑制TIMP-1表达,提高MMP-13活性与促进病理沉积间质性胶原的降解。
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| 关 键 词： | 扶正化瘀方 肾间质纤维化 胶原 基质金属蛋白酶 基质金属蛋白酶组织抑制物 |
Effect of Fuzheng Huayu Recipe on activity of matrix metalloproteinases in rats with renal interstitial fibrosis |
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| XIE Hong-dong,YANG Ke,WANG Qing-lan,TAO Yan-yan,LIU Cheng-hai,. Effect of Fuzheng Huayu Recipe on activity of matrix metalloproteinases in rats with renal interstitial fibrosis[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2010, 0(10) |
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| Authors: | XIE Hong-dong YANG Ke WANG Qing-lan TAO Yan-yan LIU Cheng-hai |
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| Affiliation: | XIE Hong-dong1,YANG Ke1,WANG Qing-lan1,TAO Yan-yan1,LIU Cheng-hai1,2,3(1Institute of Liver Diseases,Shuguang Hospital of Shanghai Chinese Medical University,Shanghai 201203,China,2Shanghai E-Institute of TCM Internal Medicine,3Ministry of Education China Liver & Kidney Key Lab,Shanghai Chinese Medical University,China) |
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| Abstract: | Objective:To investigate the action mechanism of Fuzheng Huayu Recipe(FZHY) against renal interstitial fibrosis via regulating the activity of matrix metalloproteinases.Methods:Male SD Rats were randomly divided to normal,model and FZHY treatment group.Renal interstitial fibrosis model was induced by oral administration of HgCl2 for 8 weeks,while the rats in the treatment group received FZHY.Blood serum creatinine(Cr) and blood urea nitrogen(Bun) content were determined with kits;HydroxyProline(Hyp) content... |
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