柴芍承气汤对实验性重症急性胰腺炎大鼠肠黏膜屏障的保护作用

目的 观察中药柴芍承气汤对实验性重症急性胰腺炎大鼠肠黏膜屏障的保护作用.方法 用10%左旋精氨酸溶液腹腔注射制作重症急性胰腺炎大鼠模型,将72只SD大鼠随机分为正常对照组,SAP模型组和中药治疗组.中药治疗组于造模完成后灌服(1 ml/100 g体重)中药1次,后同剂量每6 h一次,直至处死;正常对照组和SAP模型组仅灌服同等剂量的无菌生理盐水.各组分别在6、12、24、48 h取材并分别检测:①胰腺和回肠黏膜病理变化;②回肠黏膜细胞凋亡指数;③检测血清TNF-α水平.结果 中药治疗组各观察时相点胰腺和回肠黏膜病理损害均较SAP组减轻;SAP组肠黏膜细胞凋亡指数显著高于正常对照组和中药治疗组(P＜0.05);SAP组血清TNF-α含量显著高于正常对照组和中药治疗组(P＜0.05);中药治疗组血清TNF-α的含量显著高于正常对照组(P＜0.05).结论 肠黏膜细胞的过度凋亡是SAP肠道屏障功能异常的主要机制之一,柴芍承气汤能有效抑制SAP大鼠肠黏膜细胞的过度凋亡,改善肠道屏障功能.

The Protection of Chaishao Chengqi decoction to Intestinal Mucosal Barrier in Rats with severe acute pancreatitis

Objective To observe the protection of Chaishao Chengqi decoction to the intestinal mucosal barrier in rats with severe acute pancreatitis.Methods 72 SD rats were randomly divided into normal control group,SAP group and Chaishao Chengqi decoction therapeutic group.10%L-arginine was injected into the rats abdominal to establish SAP models. Chaishao Chengqi decoction 1ml.kg-1 was injected into the rats' stomach of therapeutic group and repeated injections were given per 6 hours until the rats were executed. Normal control group and SAP group were given isodose stroke-physiological saline solution. Specimens were get at 6,12,24 and 48h for detections:①Pathological change of pancreas and Ileal mucous membrane;②Apoptotic index of Ileal mucous membrane;③TNF-α contents of blood serum. Results Apoptotic index of Ileal mucous membrane in SAP group was significantly higher than Normal control group and therapeutic group(P＜0.05);TNF-α contents of SAP group was significantly higher than normal control group and theraroup(P＜0.05);Pathological lesion of pancreas and Ileal mucous membrane of therapeutic group was slighter than SAP group.Conclusions Our experiment suggested that excessus apoptosis of intestinal mucosa was one of the main mechanisms of mucosal barrier function disorder in SAP. Chaishao Chengqi decoction has a protective effect on the intestinal mucosal barrier function.