早期大鼠实验性糖尿病视网膜病变模型的建立及观察

目的建立链脲佐菌素（STZ）诱导的糖尿病大鼠视网膜病变病理模型并评价其应用价值.方法34只封闭群Wistar大鼠分为正常对照组（CON）、糖尿病3个月组（DM3）和糖尿病6个月组（DM6）,采用化学药物STZ大剂量一次性腹腔注射建立糖尿病大鼠模型,观察各组大鼠一般生理指标,并对糖尿病模型大鼠进行视网膜组织病理学检查及血管内皮生长因子（VEGF）免疫组化检测.结果STZ腹腔注射大鼠成模率100%,DM3组及DM6组大鼠视网膜均出现程度不等的水肿、血管扩张和细胞排列紊乱等改变,DM6组更加显著.视网膜VEGF表达阳性细胞率在DM3组为38%,DM6组为89%.结论STZ诱导的糖尿病大鼠在病程6个月以上时可作为早期类似人类背景型糖尿病视网膜病变的模型.

Establishment and evaluation of experimental early- stage diabetic retinopathy in rat

Objective To establish a model of early diabetic retinopathy in rat and study the retinal changes by histopathology.?Methods 34 Wistar rats were divided into three groups: CON(normal control),DM3(diabetes for 3 months) and DM6(diabetes for 6 months).The diabetic rat model was induced by intraperitoneal injection of streptozotocin(STZ).The retinal pathology and the expression of vascular endothelial growth factor(VEGF) in retina were studied.?Results Diabetic model was successfully induced in all rats with STZ ip.Retinal lesions of varied degrees were found in DM3 and DM6 groups.The retina edema,microangium expansion and disorder of celluar arrangement were more severe in DM6 than in DM3 group.The positive rate of retinal VEGF expression in DM3 and DM6 groups was 38% and 89%,respectively.?Conclusion The diabetic retinopathy model in rat with STZ-induced diabetes for 6 months,may represent the early stage of human background diabetic retinopathy.