抗氧化剂及非甾体抗炎药物对大鼠阿尔茨海默病的预保护研究

目的研究预先施加抗氧化剂VirC和VitE及非甾体抗炎药Ibuprofen对阿尔茨海默病(AD)大鼠模型认知功能的预保护作用。方法 SD大鼠72只按体重随机分为6组:其中3个药物干预组包括VitC+VitE组、Ibuprofen组和VitC+VitE+Ibuprofen组;另3组分别为直接造模组、空白对照组和生理盐水组,每组12只。连续给药15周后,分别对各组大鼠进行AD造模。进行Morris水迷宫行为学测试、免疫组织化学染色及海马CA3区病理特征观察。结果与空白对照组和生理盐水组比较,药物干预组和直接造模组大鼠的逃避潜伏期延长,平台所在象限游泳时间缩短,平台所在象限游泳路径长度占总路径长度的百分比降低(P<0.05,P<0.01);空白对照组脑源性神经营养因子阳性细胞率高于其他组(P<0.01),直接造模组低于其他组(P<0.05,P<0.01),VitC+VitE+Ibuprofen组高于VitC+VitE组和Ibuprofen组(P<0.05)。海马CA3区神经细胞受损严重,直接造模组改变最为明显。结论抗氧化剂及非甾体抗炎药物有一定的神经保护作用,可在一定程度上抵抗AD造模对大脑的损害,两者联合使用效果更加显著。

Study on preventing Alzheimer disease via antioxidant and NSAID

Objective To study the effects of antioxidant agent(AO) VitC and VitE and non-ster-oidal anti-inflammatory drug(NSAID) ibuprofen on preventing rats from Alzheimer disease(AD). Methods Seventy-two SD rats were randomly divided into 6 groups by weight:3 of 6 groups were respectively given VitC+VitE, ibuprofen and VitC+VitE+ibuprofen as medicine pre-intervention groups before AD model establishment;1 group as direct AD model group,1 as control and 1 as normal saline group. Each group had 12 rats. Rat models were established after successive drug administration for 15 weeks. Morris water maze test was performed, brain-derived neurotrophic factor(BDNF) levels in hippocampus were measured and pathologic changes were observed. Results Compared with the control and normal saline groups, the rats in medicine intervention groups and direct AD model group showed prolonged escape latency, shortening of time spent in platform's quadrant swimming, decrease in the proportion of swimming distance in platform's quadrant in total swimming tract of searching for platform. The BDNF level of the control group was higher than that of the other groups,BDNF of the direct AD model group was lower than that of the other groups,and that of the VitC+VitE+Ibuprofen group was higher than that of either VitC+VitE group or ibuprofen group (P<0.05). Neurons in CA3 area of hippocampus were found to be destroyed,and the destruction was most severe in direct AD model group. Conclusion Both AO and NSAID are effective for preventing rats from the brain impairment resulting from establishing AD model,especially when the two kinds of drugs were administrated together.