急性高眼压大鼠视网膜RhoA的分布及表达

目的:探讨急性高眼压大鼠视网膜RhoA的分布及表达变化。方法:将56只SD大鼠随机分成正常对照组、急性高眼压后1,3,7d共4组,用免疫组化和半定量RT-PCR方法检测大鼠视网膜组织中的RhoA的分布及表达情况。结果:正常及急性高眼压后1d,RhoA主要分布于视网膜神经纤维层及神经节细胞层;3d分布于神经节细胞层及内丛状层;7d分布于神经节细胞层、内丛状层、内核层及外丛状层。半定量RT-PCR提示:在正常大鼠视网膜组织中,RhoA mRNA仅有微量表达,急性高眼压后1,3,7d,RhoA表达均显著高于正常组(P<0.05),7d组表达量最高。结论:大鼠急性高眼压损伤后,视网膜RhoA蛋白的分布及表达显著增加,其在介导抑制性信号阻断轴突再生的抑制过程中发挥着重要作用。

Distribution and expression of RhoA in rat retina after acute high intraocular pressure

AIM:To investigate the distribution and expression of RhoA in the rat retina after acute high intraocular pressure. ·METHODS:Totally 56 SD rats were equally divided into 4 groups:normal group,1 day,3 days,7 days group after establishing acute high intraocular pressure models. The distribution of RhoA in the retina was assayed by immunohistochemistry and the expression was analyzed by semi-quantitative RT-PCR. ·RESULTS:In normal retina and 1 day after acute high intraocular pressure (AHIOP),RhoA was mainly distributed in retinal ganglion cell layer(GCL).In the group of 3 days after AHIOP,it existed in GCL and inner plexiform layer(IPL). However,it was detected not only in GCL and IPL but also in the inner nuclear layer(INL) and outer plexiform layer(OPL)in the group of 7 days after AHIOP. RT-PCR showed that expression of RhoA mRNA was minimal. However,the expression of RhoA in three groups of AHIOP was increased significantly than normal group (P<0.05). ·CONCLUSION:The distribution and expression of RhoA are extended and enhanced after AHIOP,RhoA play an important rule in the course of inhibiting axon regrowth.