| 高效液相色谱法测定复方曲马多片含量 |
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| 引用本文： | 常翠,杨宏图,董淳,徐群英.高效液相色谱法测定复方曲马多片含量[J].中国药房,2003,14(2):116-117. |
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| 作者姓名： | 常翠 杨宏图 董淳 徐群英 |
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| 作者单位： | 深圳市人民医院药学部,深圳市 518020 |
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| 摘 要： | 目的 :应用高效液相色谱法测定复方曲马多片含量。方法 :采用KromasilC18 柱 ,以非那西丁为内标 ,甲醇 -水 -三乙胺(55∶45∶0 2)为流动相 (冰醋酸调pH至4 2) ,检测波长为267nm ,流速0 7ml/min。结果 :曲马多线性范围为50～500μg/ml(r=0.9999 ,n=5) ;奈福泮线性范围为50～500μg/ml(r=0 9999 ,n=5)。曲马多平均回收率为99.69 % ,含量测定结果为100 71 % ,RSD=0.76 % (n=5) ;奈福泮平均回收率为99.76 % ,含量测定结果为99.64 % ,RSD=0.62 % (n=5)。结论 :本方法简便、快速、灵敏 ,结果准确。
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| 关 键 词： | 高效液相色谱法 曲马多 奈福泮 含量测定 |
| 文章编号： | 1001-0408(2003)02-0116-02 |
| 修稿时间： | 2002年3月12日 |
Determination of the Content of Compound Tramadol Tablets by HPLC |
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| CHANG Cui,YANG Hongtu,DONG Chun,XU Qunying.Determination of the Content of Compound Tramadol Tablets by HPLC[J].China Pharmacy,2003,14(2):116-117. |
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| Authors: | CHANG Cui YANG Hongtu DONG Chun XU Qunying |
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| Abstract: | OBJECTIVE:To establish a HPLC method to determine the content of compound tramadol tablets METHODS:Stationary phase:Kromasil C18 column,internal standard:phenacetin,mobile phase:methanol-water-triethlamine(55∶45∶0 2),adjusting pH to 4 2 with acetic acid,flow rate∶0 7ml/min,detecting wavelength∶267nm RESULTS:The linear range of tramadol (TR) was 50～500μg/ml(r=0 9 999,n=5) The linear range of nefopam(NFP) was 50～500μg/ml(r=0 9 999,n=5) The average recovery of TR was 99 69% The average content of TR was 100 71%,RSD=0 76%(n=5) The average recovery of NFP was 99 76% The average content of NFP was 99 64%,RSD=0 62%(n=5) CONCLUSION:The method is accurate,rapid and simple |
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| Keywords: | HPLC tramadol nefopam content determination |
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