pH值对川芎嗪在豚鼠心肌电生理-机械特性的影响中的作用

目的：观察调节ｐＨ至７．４的川芎嗪与不调节ｐＨ的川芎嗪对豚鼠心室乳头状肌电生理－机械特性的影响。方法：常规电生理方法，标准玻璃微电极技术。结果：①调节 ｐＨ至 ７．４与不调节 ｐＨ的低浓度川芎嗪（３．０～ ３０μｍｏｌ）均使豚鼠乳头状肌慢反应电位（ＳＡＰ）的动作电位幅值（ＡＰＡ），动作电位时程（ＡＰＤ），最大除极速度Ｖｍａｘ及收缩力（ＦＣ）呈剂量依赖性抑制；②调节ｐＨ至７．４的高浓度川芎嗪（０．１～３．０ ｍｍｏｌ／Ｌ）使豚鼠乳头状肌 ＳＡＰ的 ＡＰＡ、ＡＰＤ、Ｖｍａｘ及ＦＣ呈剂量依赖性增强；而不调节ｐＨ的高浓度川芎嗪（０．１～３．０ ｍｍｏｌ／Ｌ）使豚鼠乳头状肌ＳＡＰ的ＡＰＡ、ＡＰＤ、Ｖｍａｘ及ＦＣ呈抑制效应。结论：①川芎嗪对豚鼠心肌电生理－机械特性有双重影响作用，即低浓度抑制，高浓度增强。②川芎嗪本身的酸性影响川芎嗪对豚鼠心肌电生理－机械特性的正性作用。

Effect of pH in action of ligustrazine on the electrophysiological and mechnical properties in guinea pig papillary muscles

Objective: The experiment studied the effects of pH in action of ligustrazine on the electrophysiological and me-chanical properties in guinea pig papillary muscles. Methods: The electrophysiological method, the standard glass microelec-trode technology. Results: It was found ① adjusting not pH and adjusting pH to 7.4 ligustrazine ( 3.0～30μmol/L) sup-pressed the action potential amplitude (APA) the action potential duration(APD). The maximal upstroke velocity Vmax of SAPinduced by histamine and force of contraction (FC) dose-dependently. ②adjusting pH to 7.4 ligustrazine (0.1～3.0mmol/L) increas APA,APD,Vmax and FC dose-dependently, adjusting not pH ligustrazine (0.1～3.0 mmol/L) suppressed APA,APD, Vmax and FC. Conclusion: These results suggested. ①ligustrazine have dual effects on myocardiun. ②pH effect action ofligustrazine on the electrophysiulogical and mechanical properties in guinea pig papillary muscles.