Potentiation of Invasive Capacity of Rat Ascites Hepatoma Cells by Transforming Growth Factor-β |
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| Authors: | Mutsuko Mukai Kiyoko Shinkai Keiko Komatsu Hitoshi Akedo |
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| Affiliation: | Department of Tumor Biochemistry, Research Institute, The Center for Adult Diseases, Osaka, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537 |
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| Abstract: | The in vitro invasive capacity of poorly invasive cells (W1), which were cloned from rat ascites hepatoma cells (AH 130), was potentiated dose- and time-dependently by pretreating the cells with transforming growth factor-β (TGF-β). This potentiation of invasive capacity was completely abolished by anti-TGF-β antibody. When the treated cells were ip inoculated into rats, the cells extensively invaded the peritoneum, and formed penetrating tumor nodules. The effect of TGF-β was reversed by subculturing the treated cells without TGF-β. The potentiation of invasive capacity by TGF-β might participate in platelet-associated enhancement of tumor cell metastasis. |
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| Keywords: | Invasion Transforming growth factor-β Mesothelial cell Ascites hepatoma cell |
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