益母草醇提组分致大鼠肾毒性病理损伤机制研究

目的:探讨长期大量给予益母草醇提组分致大鼠肾毒性的病理损伤机制。方法:按45 d毒性实验方法,进行益母草醇提组分高、中、低剂量组的肾组织病理学检查;检测大鼠血清丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)以及总巯基(-SH)的含量和活性。结果:病理组织学检查发现,益母草醇提组分高、中、低剂量组均可见不同程度的肾小管损伤,且损伤程度随剂量增加而加重,与空白组相比有明显差异;血清酶学检测发现,益母草醇提组分可导致MDA、总-SH含量增加,SOD,GSH-Px活性下降,GSH含量降低,上述变化随剂量的增加而逐渐加重,与空白组相比有明显差异。结论:氧化损伤机制可能是益母草导致肾毒性病理损伤的机制之一。

Pathological Damage Mechanism of Rats' Nephrotoxicity caused by Alcohol Extracted Components of Herba Leonuri

Objective: To study the pathological damage mechanism of rats,nephrotoxicity after taking a large dose of alcohol extracted components of herba leonuri for a long time. Method: According to 45-day toxicity by experimental methods, to carry out the histopathologic examination of nephridial tissue;to detect the content of MDA,total SH,GSH and activity of SOD,GSH-Px in serum. Result: Histopathological examination shows: the high, medium and low dose group of alcohol extracted components of herba leonuri can induce the different degrees of renal tubular injury,and the damage gradually aggravates with dose increasing; can induce the content of total-SH,MDA in serum increasing, GSH in serum decreasing and the activity of SOD,GSH-Px decreasing in serum, and the above-mentioned change gradually aggravates with dose increasing, and it is the obvious discrepancy compared with control group. Conclusion: The oxidative damage mechanism may be one of the pathological damage mechanisms caused by Herba Leonuri.