病理性瘢痕中p53和cyclin D1的mRNA表达的实验研究

目的　探讨病理性瘢痕中p5 3、cyclinD1的表达及在瘢痕疙瘩与增生性瘢痕中表达的区别,以进一步了解瘢痕疙瘩与增生性瘢痕的形成及发展的机理。方法　手术切取病理性瘢痕标本作为实验组,以普通瘢痕作为对照组,运用RT -PCR方法测定病理性瘢痕中p5 3和cyclinD1的mRNA表达情况。结果　病理性瘢痕中p5 3和cyclinD1的mRNA表达与对照组比较差异有显著意义(P <0 .0 5 ) ;而瘢痕疙瘩中p5 3的mRNA表达明显高于增生性瘢痕,两者比较差异有显著意义(P <0 .0 5 )。结论　病理性瘢痕中p5 3和cyclinD1的异常表达,在病理性瘢痕的形成及发展中起重要作用。

Study on the expression and distribution of p53 and cyclin D_1 mRNA in pathological scar

Objective To investigate p53 and cyclin D_1 expression and their difference in keloid and hypertrophic scars, explore the formative and developmental mechanism of the keloid and hypertrophic scar. Methods The incising scar specimen as experimental group, the normal scar as the control group, evaluate the expression and distribution of p53 and cyclin D_1 mRNA with RT-PCR. Results The expression of p53 and cyclin D_1 mRNA in pathological scar is significantly different from the control group (P<0.05); The expression of p53 mRNA is higher in keloid than it in the hypertrophic scar (P<0.05). Conclusion Abnormal expression of p53 and cyclin D_1 play an important role in the formation and development of the pathological scar.