| 多重PCR检测临床分离金黄色葡萄球菌lukS/F-PV和mecA基因 |
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| 引用本文： | 闫中强,沈定霞,罗燕萍,曹敬荣.多重PCR检测临床分离金黄色葡萄球菌lukS/F-PV和mecA基因[J].中国抗感染化疗杂志,2008,8(4). |
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| 作者姓名： | 闫中强 沈定霞 罗燕萍 曹敬荣 |
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| 作者单位： | 解放军总医院微生物科 |
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| 摘 要： | 目的建立一种新的多重PCR方法,检测临床分离金葡菌的杀白细胞素毒力基因(lukS/F-PV)和甲氧西林耐药基因(mecA)。方法收集我院2006年1—12月从临床多种标本中分离鉴定的不重复金葡菌,应用多重PCR技术,优化反应条件,同时扩增葡萄球菌属特异性基因16S rRNA、金葡菌种特异性基因nuc、lukS/F-PV基因和mecA基因,扩增产物经凝胶成像系统分析。结果217株金葡菌经多重PCR检测,31株是16S rRNA+nuc+lukS/F-PV+mecA基因型,3株是16SrRNA+nuc+lukS/F-PV基因型,135株是16S rRNA+nuc+mecA基因型,40株是16S rRNA+nuc基因型,其中5株仅扩增出16S rRNA基因,3株仅扩增出nuc基因。lukS/F-PV基因和mecA基因测序显示与GenBank中的已有序列具有高度同源性,其阳性率分别为15.7%(34/217)和76.5%(166/217);34株lukS/F-PV基因阳性的分离株中,31株为MRSA,占91.2%(31/34)。结论本方法适用于快速检测和鉴定产杀白细胞素MRSA,lukS/F-PV基因阳性的菌株以MR...
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| 关 键 词： | 金葡菌 杀白细胞素毒力基因 mecA基因 聚合酶链反应 |
Multiplex PCR for detection of lukS/F-PV and mecA genes in Staphylococcus aureus isolates |
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| YAN Zhong-qiang; SHEN Ding-xia; LUO Yan-ping; CAO Jing-rong.Multiplex PCR for detection of lukS/F-PV and mecA genes in Staphylococcus aureus isolates[J].Chinese Journal of Infection and Chemotherapy,2008,8(4). |
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| Authors: | YAN Zhong-qiang; SHEN Ding-xia; LUO Yan-ping; CAO Jing-rong |
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| Institution: | YAN Zhong-qiang; SHEN Ding-xia; LUO Yan-ping; CAO Jing-rong.(Department of Clinical Microbiology; General Hospital of PLA; Beijing 100853; China); |
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| Abstract: | Objective To develop a novel multiplex polymerase chain reaction(M-PCR) assay for detection of Panton-Valentine leukocidin virulence genes(lukS/F-PV) and methicillin resistant gene(mecA) in Staphylococcus aureus.Methods A total of 217 non-duplicate strains of Staphylococcus aureus were isolated in our hospital from January 2006 to December 2006.All isolates were subject to the novel M-PCR to detect the Staphylococcus genus-specific 16S rRNA gene,the S.aureus species-specific nuc gene,the lukS/F-PV genes(whi... |
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| Keywords: | Staphylococcus aureus Panton-Valentine leukocidin virulence genes mecA gene Polymerase chain reaction |
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