我国健康成人血清中抗成人腹泻轮状病毒抗体调查

本文报告以未知血清作为第一抗体直接包被微量凹井板,用双抗体夹心ELISA法检测抗成人腹泻轮状病毒抗体,得到成功的结果。此法应用于检测从中国一些省市收集的1,380份健康成人血清中抗成人腹泻轮状病毒抗体,其中141份阳性,总阳性率为10.2%,各地的感染率不同。对25份阳性血清进行了阻断试验,结果是在阻断后的P/N比值全部低于阻断前的P/N比值。这25份阳性血清中有17份来自河北省迁安县一带农村,该地区在两年前曾发生成人流行性腹泻。西藏50份血清均阴性,海南岛50份血清中仅1份阳性。

Investigations on the Antibody against Adult Diarrhoea Rotavirus among Healthy Population in China

This article deals with a method of using unknown serum as the first antibody to coat wells of the microtiter plate directly in app lication of the sandwich ELISA method with double antibodies for assay of the antibody against adult diarrhoea rotavirus. Successful results were obtained. This method was applied for assay of the antibody against adult diarrhoea rotavirus in a total of 1,380 sera of healthy adults obtained from some provinces and. cities of China, of which 141 showed a positive result with a total positive rate of 10.2%. The infection rate varied in different regions. A blocking test was carried out on 25 positive sera the results of which revealed that P/N ratios after blocking were all lower than those before blocking. Seventeen of these 25 positive serum specimens were obtained from rural areas of Qian''an County, Hebei Province where adult epidemic diarrhoea had occurred two years ago. The antibody against adult diarrhoea rotavirus was not detected in 50 adult sera from Xizang Autonomous Region. Only one out of 50 adult sera from Hainan Island was positive for the antibody.