HPLC 法同时测定复方血栓通胶囊中5种皂苷类成分

目的：建立 HPLC 法同时测定复方血栓通胶囊中人参皂苷 Rg1、Re、Rb1、Rd 和三七皂苷 R1含量的方法。方法采用YMC －Pack Pro C18柱（250 mm ×4．6 mm，5μm），以乙腈为流动相 A，水为流动相 B 进行梯度洗脱，检测波长为203 nm，流速为1．0 mL·min －1，柱温为35℃。结果测得三七皂苷 R1在50．10～501．00 ng、人参皂苷 Rg1在151．02～1510．20 ng、人参皂苷Re 在32．01～302．10 ng、人参皂苷 Rb1在102．23～1022．30 ng、人参皂苷 Rd 在16．32～163．20 ng 范围内线性关系良好（r ＝0．9999）；精密度、稳定性、重复性 RSD 均小于3％；平均加样收回率在95％～105％之间。结论该法简便、准确、重复性好，可用于复方血栓通胶囊的质量控制。

Determination of five saponins compounds in compound Xueshuantong capsule by HPLC

Objective To establish an HPLC method for determination of Notoginsenoside R1 ,Ginsenosides Rg1 ,Ginsenosides Re,Gin-senosides Rb1 ,Ginsenosides Rd in Compound Xueshuantong Capsule by HPLC.Methods The compounds were separated by gradient elution on YMC-Pack Pro C18 column(250 mm ×4.6 mm,5 μm)as stationary phase;acetonitrile and water were adopted as gradient mixed mobile phase.The detection wavelength was set at 203 nm and the column temperature was 35℃,with the flow rate of 1.0 mL· min -1 .Results Notoginsenoside R1 ,Ginsenosides Rg1 ,Ginsenosides Re,Ginsenosides Rb1 ,Ginsenosides Rd all had good linear rela-tion(r =0.999 9)in the ranges of 50.10 ~501.00 ng,151.02 ~1 510.20 ng,32.01 ~302.10 ng,102.23 ~1 022.30 ng,16.32 ~163.20 ng,respectively.RSD of precision,stability and repeatability were lower than 3 %.The average recovery rates were in the range of 95% ~105%.Conclusions The method is simple,accurate,reproducible and suitable for the quality control of Compound Xue-shuantong Capsule.