芪苓益肝颗粒对肝硬化再损伤大鼠的保护作用

目的：探讨芪苓益肝颗粒对肝硬化大鼠再损伤的保护作用。方法：20%四氯化碳（CCl4）腹腔注射联合1.2%小牛血清蛋白（BSA）皮下注射制备肝硬化大鼠模型，成模后分区、分组，其中部分模型继续予 CCl4及 BSA损伤肝脏，分别予芪苓益肝颗粒及复方鳖甲软肝片溶液灌胃，全部灌胃5周，于14周末麻醉杀检。检测基质金属蛋白酶抑制因子-1（Tissue inhibitors of metalloproteinase-1，TIMP-1）、基质金属蛋白酶-2（Matrix-metalloproteinases-2，MMPS-2）、羟脯氨酸（Hydroxyproline，Hyp）、III型前胶原（Procollagen III，PCIII）、透明质酸（Hyaluronic Acid，HA）、层粘连蛋白（Laminin，LN）、IV型胶原（Collagen Type IV， C-IV）、肝脏病理等。结果：与模型组比较芪苓益肝颗粒能有效降低大鼠血清中TIMP-1、MMPS-2、Hyp表达（P＝0.039，0.023，0.020），降低肝组织匀浆中 PCIII、HA、LN、C-IV 的含量（P〈0.05）；继续予损伤因素的肝硬化模型鼠检测指标均有上升趋势；芪苓益肝颗粒组与复方鳖甲软肝片组在各项指标检测中无显著差异。结论：芪苓益肝颗粒对肝硬化大鼠具有干预保护作用。

The protective effect of Qiling Yigan granule against the liver cirrhosis re-damaged rats

Objective： This article researches the protective effect of Qiling Yigan granule to the injured rat, and also its influence to these cirrhosis rats. Methods： 20% CC14 injected through abdominal cavity and 1.2% BSA injected by subcutaneous were used to establish the liver cirrhosis model and then divided into groups. Qiling Yigan granule and solution of compound turtle shell soft liver piece were gavaged individually. Besides, some of the rat models were continuing damaged by CC14 and BSA, then were anesthetized and killed to inspection after 5 weeks, measuring fibrosis of liver index, such as tissue inhibitors of metalloproteinase-1, matfix-metalloproteinases-2, hydroxyproline etc. Results： Compared with the model group, QilingYigan granule are benefit to reducing the measuring index （P ~ 0.039, 0.023, 0.020）, which have the statistic meanings according to （P〈0.05）; The index of cirrhosis rat model continuing damaged by CC14 and BSA has the tendency to rise; Qiling Yigan granule and compound turtle shell soft liver piece have no significant difference in measured index. Conclusion： Qiling Yigan granule plays a protecting role in the re-damaged rats.