秦岭冷杉茎叶的化学成分研究

目的研究秦岭冷杉Abies chensiensis茎叶的化学成分。方法利用大孔吸附树脂、葡聚糖凝胶、硅胶柱色谱、半制备高效液相色谱等多种色谱方法分离纯化,根据理化性质及波谱数据对化合物进行结构鉴定。结果从秦岭冷杉95%乙醇提取物中分离得到24个化合物,分别鉴定为7,14,24-mariesatrien-26,23-olide-3α,23-diol(1)、(23R)-3α-hydroxy-9,19-cyclo-9β-lanost-24-en-26,23-olide(2)、7-oxocallitrisicacid(3)、23-hydroxy-3-oxomariesia-8(9),14,24-trien-26,23-olide(4)、紫果冷杉三萜E(5)、3-oxo-9β-lanosta-7,24-dien-26,23R-olide(6)、7,14,22Z,24-mariesatetraen-26,23-olide-3-one(7)、(+)-rel-3α-hydroxy-23-oxocycloart-25 (27)-en-26-oic acid(8)、cycloart-25-en-3β,24-diol(9)、紫果冷杉三萜H(10)、3-oxo-24,25,26,27-tetranolanost-8-en-23-oic acid(11)、abiesadine C(12)、13β-epidioxy-8(14)-abieten-18-oic acid(13)、dehydroabietic acid(14)、15-hydroxydehydroabietic acid(15)、methyl 18-methoxydehydroabietate(16)、7β-hydroxy dehydroabietic acid(17)、dehydroabietan-18-ol(18)、centdaroic acid(19)、abietic acid(20)、6,8,11,13-abi-etatrien-18-oic acid(21)、abiesadine B(22)、abiesadine N(23)、12β-hydroxyabietic acid(24)。结论所有化合物均为首次从该植物中分离得到,其中化合物11为属内首次分离得到。

Study on chemical constituents of Abies chensiensis

Objective To study the chemical constituents of the sterns and leaves parts of Abies chensiensis. Methods The isolation and purification were carried out by HP-20 macroporous resin, Sephadex LH-20, silica gel column chromatography, semi-preparative HPLC. Their structures were elucidated on the basis of physicochemical properties and spectroscopic data. Results Twenty-four compounds were isolated and elucidated from the sterns and leaves parts of A. chensiensis, their structures were identified as 7,14,24-mariesatrien-26,23-olide-3α,23-diol(1),(23 R)-3α-hydroxy-9,19-cyclo-9β-lanost-24-en-26,23-olide(2), 7-oxocallitrisic acid(3), 23-hydroxy-3-oxomariesia-8(9),14,24-trien-26,23-olide(4), neoabiestrine E(5), 3-oxo-9β-lanosta-7,24-dien-26,23 R-olide(6), 7,14,22 Z,24-mariesatetraen-26,23-olide-3-one(7),(+)-rel-3α-hydroxy-23-oxocycloart-25(27)-en-26-oic acid(8), cycloart-25-en-3β,24-diol(9), neoabiestrine H(10), 3-oxo-24,25,26,27-tetranolanost-8-en-23-oic acid(11), abiesadine C(12), 13β-epidioxy-8(14)-abieten-18-oic acid(13), dehydroabietic acid(14), 15-hydroxydehydroabietic acid(15), methyl 18-methoxydehydroabietate(16), 7β-hydroxy dehydroabietic acid(17), dehydroabietan-18-ol(18), centdaroic acid(19), abietic acid(20), 6,8,11,13-abi-etatrien-18-oic acid(21), abiesadine B(22), abiesadine N(23), 12β-hydroxyabietic acid(24). Conclusion All compounds are isolated from the plants of A. chensiensis for the first time, and compound 11 is isolated from the Abies genus for the first time.