Investigations on Carnitine Derivatives in Human Seminal Fluid |
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Authors: | J. Frankel L. M. Lewin |
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Affiliation: | Dept. of Chemical Pathology, Sackler Medical School, Tel Aviv University, Tel Hashomer, Israel |
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Abstract: | Free L-carnitine and its derivatives, present in human seminal fluid, were fractionated on Dowex 50WX8 (H +) cation exchange columns by sequential elution with HCl (1 M, 2.5 M and 4 M). Free L-carnitine eluted with 1 M HCl, whereas other carnitine-containing materials eluted with 2.5 M and 4 M HCl. Chromatography of semen extracts on Whatman No 1 paper likewise resolved three classes of materials which satisfied the carnitine requirement of Torulopsis bovina ATCC 26014. In addition to water soluble alkali-hydrolyzable materials with the properties of short chain acylcarnitines, there was another carnitine derivative, resistant to hydrolysis, which eluted from Dowex 50 W columns with 4 M HCl. It was concluded the human semen contains free carnitine. short chain acylcarnitines and an unidentified highly polar carnitine derivative. |
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Keywords: | Carnitine acylcarnitines semen seminal fluid |
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