The inhibition of tumor cell adhesion on human mesothelial cells (HOMC) by phospholipids in vitro |
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| Authors: | M. Jansen P. Lynen Jansen J. Otto T. Kirtil S. Neuss K.-H. Treutner V. Schumpelick |
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| Affiliation: | (1) Department of Surgery, University Clinic, RWTH Aachen, Pauwelsstr. 30, 52057 Aachen, Germany;(2) Interdisciplinary Centre of Clinical Research (IZKF) Biomat, University Clinic, RWTH Aachen, Pauwelsstr. 30, 52057 Aachen, Germany;(3) Institute of Pathology, University Clinic, RWTH Aachen, Pauwelsstr. 30, 52057 Aachen, Germany |
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| Abstract: | Background and aims Intraperitoneal tumor cell adhesion to extracellular matrix and to mesothelial cells mediated by integrins is an important step in developing peritoneal carcinosis. In former animal studies, we could demonstrate that intraperitoneal treatment with a new phospholipid (PL) emulsion significantly reduces the amount of peritoneal carcinosis by adhesion prevention. This in vitro study tries to elucidate the influence of phospholipids on cells of the human gastric cancer cell line (NUGC-4) and the human rectal cancer cell line (HRT-18) adhering to mesothelial cells (HOMC) in a monolayer culture in vitro. Materials and methods HOMC cells were derived from omentum majus from patients undergoing elective abdominal surgery. Three passages of both cancer cell lines (NUGC-4 and HRT-18) were used. 1×105/100 μl (HRT-18) or 1.2×105/100 μl (NUGC-4) cells, according to forgoing dilution series, were pretreated with different concentrations of phospholipid emulsion (0.05, 0.1, 0.5, 0.75, 1% PL) stained with cell tracker chloromethyl-benzamidodialkylcarbocyanine (CM-DIL) and seeded into each well on the mesothelial monolayer. After 90 min, the number of adherent cells was counted by fluorescence microscopy at 530 and 620 nm. Additionally, flow cytometric analysis of integrin α3 and β1 expression on the tumor cell surface after treatment with phospholipids was completed. Results We found a dose dependent effect of phospholipids on both tumor cell lines causing a reduction of cell–cell adhesion. Already low concentrations of phospholipids (PL 0.5) had a significant influence. The mean cell count could be reduced from 234±12/mm2 in controls to 124±41/mm2 (PL 0.5; NUG-4) and from 295±49/mm2 to 169±29/mm2 (PL 0.5; HRT-18), respectively. Additionally, the integrin α3 and β1 expression on both cell lines could be reduced. Conclusion Our results within the scope of published data indicate that adhesion prevention is capable to reduce peritoneal carcinosis. Best of Forum Papers presented at the Annual Meeting of the German Society of Surgery, 2–5 May 2006, Berlin, Germany |
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| Keywords: | Tumor cell adhesion HOMC Integrins In vitro Phospholipids |
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