慢性阻塞性肺疾病患者呼出气冷凝液中白细胞三烯B4和肿瘤坏死因子-α的检测及其临床意义

目的 观察慢性阻塞性肺疾病(COPD)患者呼出气冷凝液(EBC)中自细胞三烯B4(LTB4)和肿瘤坏死因子-?TNF-?浓度的改变及临床意义.方法 收集20例COPD患者急性发作期和缓解期以及同期20例健康体检者(健康对照组)的EBC,检测EBC中LTB4和TNF-?同时检测COPD患者的1秒用力呼气容积(FEVl)、最大呼气流量(PEF)、pH值、氧合指数(PaO2/FiO2)、动脉血氧分压(PaO2)及血白细胞(WBC)计数.结果 COPD患者急性发作期和缓解期LTB4[(35.43±14.19)ng/L和(24.39±13.75)ng/L]均显著高于健康对照组((16.75±7.44)ng/L],而缓解期LTB4值显著低于急性发作期,差异均有统计学意义(P均<0.05).COPD患者急性发作期TNF-醄(9.35显著高于缓解期[(4.42±4.11)ng/L]和健康对照组[(4.45±3.92)ng/L],差异均有统计学意义(P均

Determination and clinical implication of leukotriene B4 and tumor necrosis factor-α in condensate of exhaled breath of chronic obstructive pulmonary disease patients

Objective To study the changes and clinical implication of leukotriene B4(LTB4) and tumor necrosis factor-α(TNF-α)in exhaled breath condensate (EBC) of chronic obstructive pulmonary disease (COPD) patients.Methods EBC of 20 patients in acute episode of COPD (AECOPD),20 patiants in period of remission of COPD,and 20 persons who were having regular check-up (healthy control group) were enrolled.The concentrations of LTB4 and TNF-α in EBC were assayed.Forced expiratory volume in one second (FEV1) and peak expiratory flow (PEF) of COPD patients were observed at the same time,pH,oxygenation index (PaO2/FiO2),partial pressure of oxygen in arterial blood (PaO2) and leukocyte count were also determined.Results ①The concentrations of LTB4 in EBC of AECOPD(35.43±14.19)ng/L and remission of COPD (24.39±13.75)ng/L,were significantly higher than that of the healthy control group (16.75±7.44)ng/L,and the concentration of LTB4 in EBC during remission of COPD was significantly lower than that of AECOPD (all P