淫羊藿次苷Ⅰ在大鼠体内的药动学和组织分布研究

 目的研究淫羊藿次苷Ⅰ在血液和各组织中的HPLC检测方法及在大鼠体内的药动学和组织分布。方法采用Dikma-C₁₈色谱柱(4.6mm×250mm,5μm),流动相甲醇-0.2%磷酸(73:27),流速1.0mL·min^-1,检测波长270nm,柱温20℃。大鼠按淫羊藿次苷Ⅰ单剂量10mg·kg^-1尾静脉注射后,采用高效液相色谱法测定给药后不同时间的淫羊藿次苷Ⅰ血药浓度和各组织浓度,用3P97程序计算药动学参数。结果淫羊藿次苷Ⅰ的血药浓度-时间曲线符合二室模型,并在心脏和肌肉组织有一定程度的蓄积。结论此方法简便、准确、稳定,可用于淫羊藿次苷Ⅰ的药动学和组织分布研究。

Study on Pharmacokinetics and Tissue Distribution of Icariside Ⅰ in Rats by HPLC-UV Assay

OBJECTIVE To establish a HPLC-UV assay for the determination of icarisideⅠ in blood and tissue samples,and to study its pharmacokinetics and tissue distribution in rats.METHODS IcarisideⅠ was analyzed on a Dikma C₁₈ column with the mobile phase consisting of methnol-0.2% phosphate acid(73∶27) at 1.0 mL·min_-1 flow rate and with column temperature at 20 ℃.The wavelength of UV detector was set at 270 nm.A single dose of 10 mg·kg^-1 icarisideⅠ was administrated intravascularly in rats.The icariside Ⅰ concentrations in blood and tissues were assayed by HPLC-UV method.The pharmacokinetic parameters of icarisideⅠ were calculated by 3P97 program.RESULTS The concentration-time curve of icarisideⅠ was fitted to a two-compartment model.IcarisideⅠ was remained in heart and muscle in some degree.CONCLUSION The method was convenient,accurate and specific.It was suitable for the study on pharmacokinetis and tissue distribution of icariside Ⅰ.