大鼠心肌肌质网DNA结合蛋白的提取及在急性气胸时的变化

目的：探讨心肌细胞肌质网是否存在DNA结合蛋白，并观察它在急性气胸时的变化。方法：Wistar大鼠随机分为对照组、急性气胸组，差速离心法分离大鼠心肌细胞和后肢骨骼肌肌纤维肌质网（sarcoplasmic reticulum,SR)膜蛋白，采用Western免疫印迹技术分离出SR DNA结合蛋白，并观察此蛋白在急性气胸时的变化特征。结果；大鼠心肌SR上存在DNA结合蛋白，相对分子质量分别为60kD。急性气胸时，此蛋白的量增加。结论：大鼠心肌SR上存在DNA结合蛋白，该DNA结合蛋白与心肌功能状态密切相关。

The Extraction of Sarcoplasmic Reticulum DNA Binding Protein in Rat Car-diac Muscle and its Alteration in Acute Pneumothorax

Objective: To study if the DNA binding proteins are discovered in the nucleus and mitochondria, and wheather they exist in sarcoplasmic reticulum. The DNA binding protein of cardiac muscle SR in rats were observed by Western and im-mounoblotting. Methods: The SR membrane proteins of cardiac muscle were obtained by centrifuging in different speed. Western immounoblotting technique was used for detecting the presence of DNA binding proteinin SR of cardiac muscle. We also investigated how the DNA binding protein changed under acute pneumothorax. Results: There were DNA binding protein in cardiac muscle SR of rats and the molecular weight which one was 78000 dalton and the other was 60000 dalton; the DNA binding protein changes in acute pneumothorax. Conclusion: The DNA binding protein of SR in rat were discovered and the DNA binding proteinincrease in acute pneumothorax. These findings corroborated an activation mechanism involving the absorbing and releasing of the Ca2+ in cardiac muscle SR of rat.