Growth and adipogenic differentiation of mesenchymal stromal bone marrow cells during culturing in 3D macroporous agarose cryogel sponges |
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| Authors: | Yu A Petrenko A Yu Petrenko L G Damshkaln N A Volkova V I Lozinsky |
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| Institution: | (1) Institute of Cryobiology and Cryomedicine Problems, National Academy of Sciences of Ukraine, Khar’kov, Ukraine;(2) A. N. Nesmeyanov Institute of Elementorganic Compounds, Russian Academy of Sciences, Moscow, Russia |
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| Abstract: | We studied the possibility of population of macroporous agarose cryogel sponges by mesenchymal stromal bone marrow cells with
their subsequent adipogenic differentiation. After 7-day culturing of mesenchymal stromal cells in agarose cryogel, the level
of cell proliferation was 35%. After 3-week culturing in a medium inducing adipogenesis we observed accumulation of intracellular
neutral lipids positively stained with Oil Red O. These findings can be used for the development of bioengineering constructions
of the adipose tissue on the basis of spongy carriers.
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 141–144, August, 2008 |
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| Keywords: | mesenchymal stromal cells macroporous agarose cryogel sponges adipogenic differentiation Alamar Blue |
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