BFGF、PDGF 对兔膝前交叉韧带重建术后早期腱骨愈合影响的实验研究

目的：探讨碱性成纤维细胞生长因子（BFGF）、血小板衍化生长因子（PDGF）对兔膝前交叉韧带（ACL）重建术后早期腱骨愈合的影响。方法选择72例健康成年新西兰兔作为研究对象，实验兔根据研究方法不同分为3组，BFGF 组、PDGF 组及对照组并进行造模，术后2、5周分别进行组织学及生物力学检测。结果 PDGF 组、BFGF 组术后2、5周的最大载荷显著高于对照组，差异有统计学意义（P ＜0．05），PDGF 组、BFGF 组术后2、5周的刚度显著高于对照组，差异有统计学意义（P ＜0．05）。结论 BFGF 及 PDGF 均能够在腱骨愈合早期通过增加兔腱骨间的间充质细胞及新生血管数量，促进炎症消散，进而促进腱骨组织的愈合。生物力学方面，BFGF 及 PDGF 能提高腱骨界面的最大刚度及负荷，增强腱骨界面的强度。

A experimental study on the effect of tendon-bone healing after anterior cruciate ligament reconstruction through BFGF and PDGF

Objective To observe the effect of tendon-bone healing after anterior cruciate ligament reconstruction through BFGF and PDGF.Methods Based of seventy two healthy matured New Zealand white rabbits underwent ACL reconstruction.Devide three groups through different methods,group BFGF,group PDGF,group normal.Results The maximum loads of group BFGF and PDGF after 2 and 5 weeks are higher than group normal,there was significant difference in maximum loads (P <0.05).The max stiffness of group BFGF and PDGF after 2 and 5 weeks are higher than group normal,there was significant difference in maximum loads(P <0.05).Conclusion The group BFGF and PDGF can promote tendon-bone healing by increasing the vascularization and blood vessels of the tendon-bone interface and vascular endothelial growth factor.In biomechanics,group BFGF and PDGF can pro-mote maximum loads、max stiffness and hardness of tendon-bone healing.