米诺环素对PC12细胞缺氧缺糖损伤后神经突生长的影响

目的：探讨米诺环素对大鼠嗜铬细胞瘤细胞（PC12）缺氧缺糖（OGD）损伤后细胞存活率及神经突生长的影响。方法分别缺氧缺糖2、4、6h及8h建立PC12细胞OGD损伤模型，将细胞分为正常对照组，OGD组和不同浓度的（0．1、1．0μM和10．0μM）米诺环素治疗组，在缺氧缺糖／复氧24h后，用CCK‐8法检测细胞存活率，免疫荧光法标记MAP‐2并在荧光显微镜下观察神经突的生长，Westernblot法检测各组GAP‐43蛋白的表达水平。结果与OGD组比较，米诺环素能显著提OGD后PC12细胞的存活率［（46．1±2．9）％vs．（77．0±2．5）％，P＜0．01］，促进PC12细胞OGD损伤后神经突的生长，同时上调轴突再生蛋白GAP‐43的表达［（0．34±0．04）vs．（2．11±0．10），P＜0．01］。结论米诺环素能减轻OGD损伤所致PC12细胞的死亡，并促进细胞神经突的生长。

Effects of minocycline on neurite outgrowth of PC12 cells following oxygen glucose deprivation

Objective To investigate the effects of minocycline on cell viability and neurite outgrowth of pheochromocytoma cells (PC12) after oxygen‐glucose deprivation(OGD) injury .Methods PC12 cells were exposed to OGD insult for 2 ,4 ,6 ,8 h to estab‐lish a cerebral ischemia model in vitro .High‐differentiated PC12 cells were cultivated and randomly divided into three groups :con‐trol group ,OGD group and various doses of minocycline(0 .1 ,1 .0 ,10 .0 μM) treated group .24 h after OGD‐reperfusion ,PC12 cells viability was assessed by CCK‐8 assay ,the neurite was labeled with MAP‐2 by immunofluorescence and neurite length was meas‐ured by the Image‐Pro Plus 7 .0 software ,GAP‐43 protein expression was determined by Western blotting .Results Compared to the OGD groups ,minocycline induced a concentration‐dependent increase in cells viability (46 .1 ± 2 .9)% vs .(77 .0 ± 2 .5)% ,P<0.01],improvedneuriteoutgrowthandincreasedtheexpressionofGAP‐43proteininPC12cellsafterOGDinjury((0.34±0.04) vs .(2 .11 ± 0 .10) ,P<0 .01] .Conclusion Minocycline could protect against oxygen glucose deprivation injury and promote neurite outgrowth .This finding suggests minocycline may be a novel therapy for cerebral ischemia .