新型稀土杂多化合物对乙型肝炎病毒复制的抑制作用

目的：研究稀土杂多化合物(PTW-6)体外抗乙型肝炎病毒（HBV）的活性。方法：MTT法检测PTW-6对Hep G2 2.2.15细胞的毒性，乙型肝炎病毒e（s）抗原诊断试剂盒检测上清液中HBeAg、HBsAg的含量，Southern blotting 法检测PTW-6对细胞内HBV DNA复制的抑制作用。荧光定量PCR分析PTW-6对细胞内HBV mRNA和上清液中HBV DNA含量的影响。结果：PTW-6对2.2.15细胞的半数中毒浓度为1590.46 mg?L^-1， PTW-6各浓度实验组对HBeAg和HBsAg的抑制率均高于对照组（P<0.05）；随着PTW-6浓度的增高，PTW-6对2.2.15细胞内外HBV DNA的抑制率增加，PTW-6对2.2.15细胞外HBV DNA和细胞内HBV mRNA半数抑制浓度分别为51.1和63.6 mg?L^-1。结论：PTW-6体外毒性较低, 且对HBV复制有较好的抑制作用。

Inhibitory effect of novel polyoxometalate on hepatitis B virus replication in vitro

Objective To evaluate the activity of novel polyoxometalate against hepatitis B virus (HBV) in vitro.Methods MTT assay was used to evaluate the growth inhibitory effects of polyoxometalate on 2.2.15 cell lines,Hepatitis B e(s) antigen(HBeAg) and Hepatitis B surface antigen in the culture medium were determined by ELISA.Southern blotting was performed to detect HBV DNA in Hep G2 2.2.15 cells.Real-time quantity PCR was applied to detect the contents of introcellular HBV mRNA and extracellular HBV DNA.Results The median toxicity concentration(TC50) was 1590.46 mg·L-1 on 2.2.15 cells.The inhibitory rats of PTW-6 on HBeAg and HBsAg were markedly higher compared with control group(P<0.05).The median inhibitory concentration(IC50) of PTE-6 in extracellular HBV DNA and introcellular HBV mRNA were 51.1 and 63.6 mg·L-1,respectively.Conclusion Novel polyoxometalate is a potential compound for treatment chronic HBV infection.