| 紫外分光光度法测定海红果中总黄酮的含量 |
| |
| 引用本文： | 赵亮,刘恩荔,李青山,梁泰刚. 紫外分光光度法测定海红果中总黄酮的含量[J]. 山西医科大学学报, 2006, 37(2): 169-171 |
| |
| 作者姓名： | 赵亮 刘恩荔 李青山 梁泰刚 |
| |
| 作者单位： | 1. 山西医科大学药学院,太原,030001
2. 山西医科大学药学院,太原,030001;山西省中医药现代工程技术研究中心 |
| |
| 基金项目： | 山西省自然科学基金;山西医科大学校科研和教改项目 |
| |
| 摘 要： | 目的建立海红果中总黄酮含量的测定方法。方法以芦丁为对照品，采用紫外分光光度法在波长500nm处测定海红果中的总黄酮。结果芦丁在0．011-0．055mg／ml浓度范围内线性关系良好，总黄酮平均回收率为100．35％，RSD1．3％（n=6）。海红干果中总黄酮含量为0．2760％。结论该方法简便、快速、准确、灵敏，可作为测定海红果中总黄酮含量的方法。
|
| 关 键 词： | 分光光度法紫外线 海红果 黄酮类 芦丁 |
| 文章编号： | 1007-6611(2006)02-0169-03 |
| 修稿时间： | 2005-11-17 |
Content determination of total flavonoids in fruit of Malus micromalus by ultraviolet spectrophotometry |
| |
| ZHAO Liang,LIU En-li,LI Qing-shan,et al. Content determination of total flavonoids in fruit of Malus micromalus by ultraviolet spectrophotometry[J]. Journal of Shanxi Medical University, 2006, 37(2): 169-171 |
| |
| Authors: | ZHAO Liang LIU En-li LI Qing-shan et al |
| |
| Abstract: | Objective To establish a method for determining the content of total flavoes in fruit of Malus micromalus(MM). Method The content of total flavonoids in fruit of MM was determined at 500 nm by ultraviolet spectrophotometry with rutin as standard control.Results The standard curve of rutin was linear in the concentration range of 0.011 to 0.055 mg/ ml.The average recovery of total flaves was 100.35% with the relative standard deviation 1.3%(n=6).The content of total flavonoids in the samples was 0.276 0%.Conclusion The method is simple,rapid and accurate.It can be used for determining the content of total flavonoids in fruit of Malus micromalus. |
| |
| Keywords: | spectrophotometry ultraviolet fruit of Malus micromalu flavoes rutin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
