| 宫颈癌患者石蜡标本中人乳头瘤病毒的检测与分型 |
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| 引用本文： | 卫星星. 宫颈癌患者石蜡标本中人乳头瘤病毒的检测与分型[J]. 长治医学院学报, 2002, 16(3): 169-171 |
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| 作者姓名： | 卫星星 |
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| 作者单位： | 长治医学院化学教研室,046000 |
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| 摘 要： | 采用非放射性同位素检测技术，对来源于子宫颈癌患者的石蜡包埋组织标本进行四种常见生殖道HPV（HPV6，11，16，18）的检测和分型。结果表明，在受检的10例宫颈癌患者石蜡标本中，HPV阳性检出率为100%，其中HPV16型3例（30%），和HPV18例6例（60%），HPV6和HPV11型未检测到，表明宫颈癌感染HPV主要为HPV16及18型。
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| 关 键 词： | 石蜡标本 分型 人乳头瘤病毒 非放射性同位素 检测 宫颈癌 |
| 文章编号： | 1006-0588(2002)03-0169-02 |
| 修稿时间： | 2002-04-05 |
Detection and Typing of Human Papillomavirus DNA in Paraffin- Embedded Cervical Carcinomas Specimens |
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| Wei Xingxing Teaching and Research Rome of Chemistry. Detection and Typing of Human Papillomavirus DNA in Paraffin- Embedded Cervical Carcinomas Specimens[J]. Journal of Changzhi Medical College, 2002, 16(3): 169-171 |
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| Authors: | Wei Xingxing Teaching and Research Rome of Chemistry |
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| Affiliation: | Wei Xingxing Teaching and Research Rome of Chemistry |
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| Abstract: | Objective:To establish a method for rapid detection and typing of human papillomavirus (HPV) DNA by nonisotopic detection.Methods:Four sequence-specific oligonucleotides of HPV DNA (types6B ?11?16?18)were used as probes and were fixed on a sheet of nylon membrane,which can be used to hybridize with the PCR-amplified target DNA-sequence,so that we can detect and identify any one of the 4 types of HPV on the same membrane.Results:10 cases of paraffin-embedded cervical carcinomas was detected and all showed positive result.Of 10 cases ,3 were positive for HPV16,making up 30%;6 for HPV18,making up 60%;1 for HPV16/18(mixed type),making up 10%;HPV6 and HPV11 were not detected.Conclusions:Cervical are mainly associated with HPV 16 and 18.The method mentioned in this article is a simple,rapid,and accurate technique for the detection and typing of HPV |
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| Keywords: | Human papillomavirus nonisotpic detection cervical cancer |
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