人食管鳞癌细胞系RJEC-2的建立及其生物学特性分析

目的:建立新的人食管鳞状细胞癌细胞系并分析其生物学特性,为食管癌分子机制和治疗干预的研究提供新的实验模型。方法:采用组织块培养法,从病人食管癌组织中分离纯化鳞状上皮癌细胞并建立细胞系。对细胞系的形态特点、细胞角蛋白表达、生长特征、细胞周期分布、细胞遗传特征和致瘤能力进行了研究分析。结果:建立了食管鳞状细胞癌细胞系RJEC-2,已在体外持续传代4个多月,传至46代,生长稳定;该细胞系具有鳞状上皮细胞的形态和特点:单层贴壁生长,免疫组化显示细胞角蛋白表达阳性,电镜下可见明显的胞质内张力纤维束和细胞间桥粒;群体倍增时间为46.5 h,细胞培养至90%汇合时,细胞周期分析显示G0/G1期平均占56.72%,S期平均占33.96%,G2/M期平均占9.32%;细胞染色体结构和数量异常,呈现肿瘤细胞特性;细胞呈克隆性生长,平板克隆平均形成率为13.93%,裸鼠移植瘤实验表明细胞具有致瘤能力,病理分析显示移植瘤与病人肿瘤病理形态特征相似,均为中、高分化鳞状细胞癌。结论:成功建立的人食管鳞癌细胞系RJEC-2,为食管癌发病机制和治疗方案的研究提供了新的研究实验模型。

Establishment and biological characterization of human esophageal squamous cell carcinoma cell line RJEC-2

Objective To establish a novel cell line of human esophageal squamous cell carcinoma(ESCC) and to investigate the biological characterization in pursuit of a new model for further studies on molecular mechanisms and therapeutic intervention.Methods Small tissue blocks taken from resected specimens of an ESCC patient were cultured,and squamous cell carcinoma cells were purified.Biological characters of the cell line were investigated,including morphology,cytokeratin expression,growth kinetic features,cell cycle,cytogenetic features and tumorigenicity.Results An ESCC cell line(RJEC-2) was established.It grew continuously in vitro for more than 4 months and 46 passages.This cell line presented features of squamous epithelial cells with growing as a monolayer,cytokeratin positive as indicated by immunohistochemical staining,tonofilaments and desmosomes under electron microscopy.The cells grew with doubling time of 45.5 h,and cell cycle distribution was 56.72% for G0/G1,33.96% for S,9.32% for G2/M phase when cells grew to 90% confluence.The cytogenentic analysis demonstrated chromosome abnormalities in both structure and number with features of tumor cells.The cell line formed colony with efficiency of 13.93% in culture plates and formed tumors in nude mice.The pathological analysis suggested that the xenograft was similar to the primary tumor and both were moderately-well differentiated squamous cell carcinoma.Conclusions We have successfully established a human ESCC cell line(RJEC-2),a new model for further study on esophageal carcinoma.