胆囊结石病人成纤维细胞生长因子-19的血浆含量及胆囊黏膜表达特点

目的:检测胆囊结石病人成纤维生长因子-19(fibroblast growth factor-19,FGF-19)的血浆含量及其在胆囊黏膜的表达量,探讨FGF-19与胆囊舒张收缩功能的关系。方法:对27例胆囊结石病人(男9例,女18例,平均年龄48.5岁),采用实时定量PCR法检测胆囊黏膜FGF-19 mRNA的表达量。采用酶联免疫吸附法测定空腹及脂餐后1.5 h、3.0 h血浆FGF-19的含量。B超检查胆囊收缩功能。结果:按脂餐后1.5 h胆囊排空率(ejection fraction,EF)将胆石病人分为胆囊收缩正常组(EF>60%,n=5),收缩减弱组[EF(30~60)%,n=15]和未收缩组(EF<0.05。结论:部分胆石病人存在胆囊收缩和舒张功能障碍。血浆FGF-19含量变化可反映胆囊收缩和舒张功能异常。

Fibroblast growth factor-19 in gallstone patients: plasma content and expression of gallbladder mucosa characteristics

Objective To evaluate the relation between fibroblast growth factor(FGF)-19 and gallbladder motility function by measuring the serum level of FGF-19 and the expression of FGF-19 mRNA in gallbladder mucosa from patients with gallstone disease.Methods Nine male and 18 female patients with gallstone disease were recruited.FGF-19 mRNA expressions in gallbladder mucosa were determined by real-time PCR.Plasma FGF-19 level at fasting,1.5 h and 3.0 h after fat diet were assayed by ELISA,the gallbladder volume at the corresponding time points were measured by B-type ultrasonic scanning.Results According to gallbladder ejection fraction(EF) at 1.5 h after fat diet,the patients were divided into 3 groups.For normal contraction group(EF > 60%,n= 5),low contraction group [EF(30 ~ 60)%,n=15] and non contraction group(EF < 30%,n=7),EF at 3.0 h after fat-diet were(67.2±3.4)%,(48.2±2.4)%,and(17.0±3.5)%,respectively,P < 0.01.Gallbladder refilling rate in normal contraction group(82±12)% was higher than low contraction and non contraction group [(37±11)% and(7±7)%],P = 0.006.FGF-19 mRNA expression in gallbladder mucosa was significantly lower in the non contraction group(130±71) than in the normal contraction group and the low contraction group(222±82 and 254±50),P<0.05.Fasting plasma FGF-19 level in non contraction group [(62.1±20.0) pg/mL] was lower than the other groups[normal contraction group:(157.9±22.3) pg/mL],low contraction group: [(175.8±25.6) pg/mL],P<0.05.Conclusions Gallbladder contraction and refilling function were impaired in subgroups of the gallstone patients.Changes of plasma FGF-19 level may reflect the abnormalities of gallbladder contraction and refilling.