应用猪小肠黏膜下层与肌腱细胞构建组织工程支架修复大鼠腹壁缺损的实验研究

目的:应用猪小肠黏膜下层(small intestinal submucosa,SIS)与肌腱细胞构建组织工程支架,研究其在修复腹壁缺损时的生物力学特性。方法:制作SD大鼠腹壁缺损模型,应用所构建的组织工程支架修补缺损,术后4周取样进行大体观察,检测组织学及力学性能。结果:组织工程支架修补术后的SD大鼠无腹部裂开及疝发生,支架与腹腔内脏器有轻微粘连;HE及Masson染色发现支架与肌肉组织交界区有显著新生血管出现及肌肉组织长入。力学性能检测显示组织工程支架的力学强度显著大于SD大鼠腹壁肌肉强度。结论:构建组织工程支架可有效修补大鼠的腹壁缺损。

Repair of abdominal wall defect with engineered scaffold using tenocytes seeded porcine small intestinal submucosa in rats

Objective To investigate the biomechanical properties of the engineered scaffold using tenocytes seeded small intestinal submucosa(SIS) in abdominal wall defect.Methods Abdominal wall defect was repaired using engineered scaffold in rat modes.The rats were sacrificed after 4 weeks and the sample tissues were analyzed by gross observation,histological method and mechanical testing.Results All rats had no abdominal dehiscence or hernia.The engineered scaffold caused slight adhesion with organs.HE and Masson staining proved the junction between SIS and surrounding tissue had more new vessels.The muscle grew into the engineered scaffold.The mechanical strength of the engineered scaffold was much more than the abdominal wall muscles,which could provide sufficient mechanical strength.Conclusions Engineered scaffold repairing abdominal wall defect is a feasible and effective method.