Quantitative analysis of angiogenesis using confocal laser scanning microscopy |
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Authors: | Linlang Guo Patricia Burke Su-Hao Lo Regina Gandour-Edwards Derick Lau |
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Institution: | (1) University of California Davis Cancer Center Sacramento, California, USA;(2) University of California Davis Cancer Center Sacramento, California, USA; Tel.: |
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Abstract: | Angiogenesis is essential for tumor growth and metastasis. Angiogenesis is commonly quantified by measuring microvessel density
(MVD) within tumors. In this report, we compared light microscopy with confocal laser scanning microscopy (CLSM) in the qualitative
and quantitative analysis of angiogenesis. MVDs were determined manually in a lung tumor xenograft and a normal skeletal muscle
using CD31 immunohistochemical staining and light microscopy. Area of three-dimensional representation of microvessels, detected
as CD31 immunofluorescence, was measured automatically using computer-assisted CLSM. By manual counting under light microscopy,
the relative level of MVD of the lung tumor vs. skeletal muscle was 0.8. However, the corresponding relative level of microvessels
was 3.4 as determined by computer-assisted CLSM. Futhermore, the architecture of microvessels was better delineated with CLSM
than with light microscopy. We have applied this CLSM method for analyzing the antiangiogenic effect of an anticancer drug,
paclitaxel, in the lung tumor xenograft model. We conclude that CLSM is an appropriate method for quantitative and qualitative
analysis of microvasculature in normal and tumor tissues.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | angiogenesis quantification confocal laser scanning microscopy |
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