大鼠骨髓间充质干细胞复合n-HA/PLA支架异位成骨的实验研究

目的]研究大鼠骨髓间充质干细胞(BMSCs)复合纳米羟基磷灰石/聚乳酸(n-HA/PLA)支架材料体内异位成骨情况.方法]取SD大鼠股骨胫骨骨髓,进行贴壁培养BMSCs,将第3代BMSCs接种到n-HA/PLA支架材料上,加入成骨诱导液,3d后置人大鼠体内,设为实验组；将单纯支架材料置入组为对照组.4、8、12周时取材进行大体和组织学观察.结果]8、12周时实验组可见类骨质成分形成,并且支架材料维持置入时形状,对照组无类骨质形成.结论]BMSCs复合n-HA/PLA构建组织工程骨有很好的异位成骨能力,且能在体内维持良好塑型.

Experimental study on ectopic bone formation induced by n-HA/PLA combined with BMSCs

Objective]To observe the ability of ectopic bone formation induced by n-HA/PLA scaffold combined with rat BMSCs.Method]BMSCs were harvested from the femur and tibia of SD rat,and obtained by using adhesive culture method.We cultured the 3rd generation BMSCs on the surface of a matrix scaffold with osteogenic medium,3 days later,the scaffolds combined with BMSCs were implanted into the rats subcutaneously(experimental group).By contrast,blank scaffolds group was implanted as control group.After 8 weeks and 12 weeks,the ability of ectopic bone formation was evaluated by gross histology observation.Result]Eight weeks and 12 weeks later,we could see maturely sclerotized in large amount in experimental group,and the scoffold maintained the shape of implanted.There was no sclerotized in control group.Conclusion]The n-HA/PLA+BMSCs has a good ability of ectopic bone formation,and the scaffold can maintain good shape in vivo.